University of Illinois

Biotechnology Research and Development Corporation

University of California

Bp Corporation North America Inc.

Zuchem, Inc.

Other

Centre National De La Recherche Scientifique

Universitéde Strasbourg

Centre National De La Recherche Scientifique-cnrs

Huimin Zhao

Ryan Woodyer

James Harrison Doudna Cate

Yong-Su Jin

William T Beeson, Iv

Wilfred A Van Der Donk

Olgica Milenkovic

Chaoguang Tian

N Louise Glass

Suk-Jin Ha

Soo Rin Kim

Nikhil Unni Nair

Jin Ho Choi

Jonathan M Galazka

Sijin Li

Xiaomin Yang

Jing Du

S M Hossein Tabatabaei Yazdi

Yongbo Yuan

Jian Ma

Michael Racine

Ryan Sullivan

Michael Simurdiak

Pierre H Chambon

John A Katzenellenbogen

Daniel Metzger

William W Metcalf

Alvaro Gonzalo Hernandez

Vinh Tran

Mingfeng Cao

Zia Fatma

Olga Esteban

Behnam Enghiad

Tyler Johnannes

Seyed Kasra Tabatabaei

The disclosure relates to a re-writable DNA-based digital storage system with a random access feature. An example embodiment includes reading a physical nucleotide sequence of 2n+L bases to form a digital representation of the physical nucleotide sequence; dividing the digital representation of the physical nucleotide sequence into an address representation of n bases, followed by a data representation of L bases, followed by a further address representation of n bases; decoding the data representation into an integer value less than 3L that is a sum of a first addend and a second addend, wherein the data representation includes a first subsequence of bases encoding the first addend followed by a second subsequence of bases encoding the second addend; and storing, in a computer memory, the integer value.

Re-writable DNA-based digital storage with random access

The present disclosure provides polynucleotides and vectors for the genomic engineering and editing of non-conventional yeasts such as. The polynucleotides and vectors can be used as tools that are efficient to alter the expression of one or more gene products in the yeast, and specifically to induce the production of organic acids or other bioproducts of interest in the yeast.

Genetic toolbox for metabolic engineering of non-conventional yeast

Nick-based methods, devices, and systems for nick-based data storage in a deoxyribonucleic acid (DNA) sequence are disclosed. Digital information is encoded in a register of at least one copy of a double-stranded DNA sequence having a plurality of nickable positions. The data is translated into a sequence of values from a nick alphabet that is subsequently mapped to the plurality of nickable positions, and the DNA sequence is nicked according to the mapped values. Because the digital information is encoded as a series of nicked and non-nicked positions of a double-stranded DNA sequence, the nucleotide sequence of the DNA can be non-synthetic, or 'native' DNA.

Nick-based data storage in native nucleic acids

Methods and compositions for specific cleavage of DNA molecules using single-stranded guides and Argonaute (Ago) proteins are described.

Programmable DNA-guided artificial restriction enzymes

The disclosure relates to a re-writable DNA-based digital storage system with a random access feature. An example embodiment includes selecting address representations of m nucleotide sequences of n bases each. Each of the address representations of m nucleotide sequences (i) consists of approximately 50% guanine and cytosine content and (ii) is self-uncorrelated. The address representations are mutually uncorrelated with one another. All of the address representations end with a particular base. The embodiment also includes selecting, for a particular address representation from the address representations of the m nucleotide sequences, a corresponding data representation of a nucleotide sequence of L bases. The embodiment further includes concatenating the particular address representation with the data representation to form a representation of a target nucleotide sequence of n+L bases. In addition, the embodiment includes synthesizing the target nucleotide sequence.

Materials and methods are described to produce xylitol from a mixture of hemicellulosic sugars by several routes. Examples include either as a direct co-product of a biorefinery or ethanol facility, or as a stand-alone product produced from an agricultural or forestry biomass feedstock including using, e.g. ethanol waste streams.

Production of xylitol from a mixture of hemicellulosic sugars

The present invention relates to a method for tightly temporally controlling the biological activity of a protein of interest in a vertebrate, upon induction of the activity of a fusion protein comprising said protein of interest and an ERM polypeptide containing a mutated ligand binding domain of the human oestrogen receptor &#x3b1;, with a synthetic ligand that does not interfere with oestrogen signalling. In particular, the present invention concerns a method for generating tightly temporally-controlled targeted somatic mutations in a vertebrate, preferably a mouse, by inducing the activity of a fusion protein comprising a site-specific recombinase protein and an ERM polypeptide, with a synthetic ligand devoid of oestrogenic and anti-oestrogenic activities.

Method for controlling the biological activity of a protein in a vertebrate cell

The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure further relates to methods of increasing transport of cellodextrin into a cell, methods of increasing growth of a cell on a medium containing cellodextrin, methods of co-fermenting cellulose-derived and hemicellulose-derived sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell, methods of increasing transport of a pentose into a cell, methods of increasing growth of a cell on a medium containing pentose sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell.

Methods and compositions for improving sugar transport, mixed sugar fermentation, and production of biofuels

Engineered mutant xylose reductases demonstrate higher preference to xylose than arabinose. Amino acid mutations were engineered in to native xylose reductase from. Mutant xylose reductases are useful in the production of xylitol and ethanol.

Xylose reductase mutants and uses thereof

Stable and active arabinitol dehydrogenases (LAD) fromand mutants thereof are disclosed. Arabinitol dehydrogenases are useful in the production of xylitol and ethanol from an arabinose containing substrate. Recombinant and heterologously expressed arabinitol dehydrogenases are useful in converting biomass into biofuels and other industrial food products.

[object Object]

A new xylose reductase encoding gene fromwas heterologously expressed inas a His-tag fusion protein and subsequently purified in high yield. This xylose reductase was shown to have a high turnover rate and catalytic efficiency, high stability at room temperature, broad pH profile, and a preference of NADPH over NADH. This enzyme is utilized in production of xylitol and other sugar alcohols such as sorbitol and also in the metabolic enhancement of organisms used for fermentation of plant biomass into ethanol.

Growing community of inventors

Champaign, IL, United States of America

Huimin Zhao