Jecho Laboratories Inc.

General Regeneratives Holdings Inc.

Shanghai Jiao Tong University

Jecho Biopharmaceuticals Co Ltd.

General Regeneratives, Ltd.

Huili Lu

Wei Han

Hua Jiang

Jianwei Zhu

Yueqing Xie

Lei Zhang

Lei Han

Junsheng Chen

Di Xiang

Andrew Burnette

Kai Ding

Cedric Cagliero

Manyu Luo

Baohong Zhang

The present disclosure discloses a method for expressing and preparing a bivalent bispecific antibody. In the present disclosure, each portion of a bivalent bispecific antibody and an immune hybrid protein thereof is respectively expressed in a suitable prokaryotic or eukaryotic cell system, separated and purified by high-performance affinity chromatography, and then spliced in vitro by trans-splicing reaction mediated by an intein, to prepare the bivalent specific antibody and an immune hybrid protein thereof.

Bivalent bispecific antibody hybrid protein expression and preparation methods

Methods for preparing a recombinant protein from a bacterium are provided. The method includes constructing an expression vector including two promoters. Each of the two promoters attaches a secretion signal peptide to one polypeptide of a protein. The protein attached with the two promoters and secretion signal peptides is then cloned into the expression vector to provide a recombinant expression plasmid. The recombinant expression plasmid is transformed into a host cell. A fermentation process is performed to grow the host cell and to induce an expression to synthesize polypeptides in the host cell and to transport the polypeptides to an outside of a cytoplasm of the host cell, such that the polypeptides are released in a soluble form in a growth medium of the host cell. The polypeptides are assembled into a three-dimensional structure of the protein. The protein is captured from the growth medium.

Method for preparing recombinant protein from bacterium and composition containing the same

CXCL9 promotes bone marrow regeneration, increases peripheral white blood cells, and increases survival if administered prior to treatment of a subject with chemotherapeutic drugs such as 5-FU or radiotherapy. Similar effects are obtained by administering an anti-CXCL9 antibody following chemotherapy or radiotherapy. Compositions and methods are presented for the treatment of cancer and bone marrow diseases.

Methods for promoting protection and regeneration of bone marrow using CXCL9 and anti-CXCL9 antibodies

CXCR3 agonists, including natural CXCR3 ligands, promote bone marrow regeneration, increase peripheral white blood cells, and increase survival if administered prior to treatment of a subject with chemotherapy or radiotherapy. Similar effects are obtained by administering an CXCR3 antagonists following chemotherapy radiotherapy. Compositions and methods are presented for the treatment of cancer and bone marrow diseases.

Methods for protecting and regenerating bone marrow using CXCR3 agonists and antagonists

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Shanghai, China

Huili Lu