Conagen Inc.

Phytogene, Inc.

Hui Chen

Xiaodan Yu

Hongxue Wang

Oliver Yu

Jixiang Han

Min Wang

Mohammad Wadud Bhuiya

Xianpeng Cai

LanLan Zhou

Provided herein are methods for making lactones comprising incubating a cellular system expressing a heterologous cytochrome P450 (CYP450) protein with a medium comprising straight chain fatty acids to produce subterminal hydroxy fatty acids, and incubating a yeast cell culture with the subterminal hydroxy fatty acids to produce lactones.

Biosynthetic production of gamma- and delta-lactones using cytochrome P450 enzymes having subterminal hydroxylase activity

The present invention relates to the production of capsaicinoid compounds including Capsaicin and Nonivamide via microbial fermentation.

Method for the microbial production of specific natural capsaicinoids

Provided herein are methods for making gamma lactones comprising reacting a carboxylic acid substrate with a heterologous cytochrome P450 (CYP450) protein with carboxylic acid 4-hydroxylase activity.

Biosynthetic production of gamma-lactones

The present disclosure relates to the production of 8-methyl nonanoic acid and medium chain branched fatty acids, e.g., via microbial fermentation.

Method for the microbial production of 8-methyl nonanoic acid

The subject technology generally relates to biosynthesis of styrene. Certain embodiments of the subject technology is based, in part, on the recognition that phenylalanine can be converted to styrene by a two-step pathway of deamination and de-carboxylation, with trans-cinnamic acid (tCA) as the intermediate. Two types of enzymes are directly involved in this process, phenylalanine ammonia lyase (PAL), which converts phenylalanine to tCA, and cinnamic acid decarboxylase, which coverts tCA to styrene. Host cells expressing these two types of enzymes can be cultured in bioreactor to produce styrene from renewable substrates such as glucose.

Enzymes and methods for styrene synthesis

Provided herein are methods of making capsinoids including providing a capsiate synthase in a mixture or cellular system, feeding 8-methyl-6-nonenoyl-CoA, 6E-8-methylnonenoic acid or 8-methylnonanoic acid into the mixture or cellular system, feeding vanillyl alcohol into the mixture or cellular system, and collecting capsinoids from the mixture or cellular system.

Methods of making capsinoids by biosynthetic processes

Disclosed are methods for ergothioneine biosynthesis. More particularly, the present disclosure relates to methods for microbial ergothioneine biosynthesis. The present disclosure relates generally to engineered host cells and methods for producing ergothioneine. More particularly, the present disclosure relates to an engineered host cell and methods for microbial ergothioneine biosynthesis using the engineered host cell.

Microbial ergothioneine biosynthesis

A biosynthetic method of making carboxyl CoA from long-chain carboxylic acid including expressing an ACS in a cellular system, feeding a long-chain carboxylic acid to the cellular system, growing the cellular system in a medium, and producing carboxyl CoA.

Methods of using acyl-CoA synthetase for biosynthetic production of acyl-CoAs

A biosynthetic method of making a capsaicinoid including expressing a first gene product of CS/AT3/Pun1 in a cellular system, growing the cellular system in a medium, and collecting the capsaicinoid. The biosynthetic method further includes expressing a second gene product of ACS1 and expressing a third gene product of pAMT in the cellular system.

Growing community of inventors

Bedford, MA, United States of America

Hui Chen