Boehringer Mannheim Gmbh

Hugo Tiedemann

Wolfgang Werner

Werner Guthlein

Peter Vogel

Walter Rittersdorf

Hans Lange

Peter Rieckmann

Bernward Sojka

Hans-Georg Rey

The detection and determination of (a) hydrogen peroxide or of a substance which reacts with the formation of hydrogen peroxide or (b) of peroxidase or a peroxidatively active substance, is carried out by contacting an unknown sample with a chromogen and, in case (a), with a peroxidatively active substance or in case (b) with hydrogen peroxide or a substance which reacts with the formation of hydrogen peroxide, and evaluating the coloration produced by the reaction, wherein, as the chromogen, there is used at least one 3,3',5,5'-tetraalkylbenzidine compound of the formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which can be the same or different, are straight-chained or branched alkyl radicals containing up to 6 and preferably up to 4 carbon atoms.

Oxidation indicators comprising 3,3',5,5'-tetraalkylbenzidine compounds

Novel 3-aminocarbazole compounds of the formula: ##SPC1## Wherein

9-Substituted 3-aminocarbazole compounds and test strip indicator

9-Substituted 3-aminocarbazole compounds

Glucose contained in urine is determined by contacting a sample with a diagnostic composition comprising an absorbent carrier impregnated with glucose oxidase, peroxidase, an indicator compound such as o-tolidine, and as a buffer, 2-(N-morpholino)-ethane-sulfonic acid.

Diagnostic composition for the determination of glucose

9-(.gamma.-AMINOPROPYL)-3-AMINOCARBAZOLE, WHEN INCORPORATED INTO INDICATOR COMPOSITIONS ALSO COMPRISING OXIDATION INDICATORS SUCH AS O-TOLIDINE FOR THE ENZYMATIC DETECTION OF GLUCOSE, RESULTS IN OUTSTANDINGLY STABLE INDICATOR COMPOSITIONS AND NOT ADVERSELY EFFECTED BY NORMALLY INTERFERING COMPONENTS IN THE TEST SAMPLE.

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Mannheim-Wallstadt, Germany

Hugo Tiedemann