Rhode Island Hospital

Circe Biomedical, Inc.

Other

W. R. Grace Co.-conn.

Hugo O Jauregui

Donna M Trenkler

Sharda Naik

Shawn P Cain

Timothy Jon Perlman

Kerry Ann Gagnon

Jin Liu

Christine Marie Tosone

Henry A Santangini

Claudy Jean-Paul Mullon

Claudy Jean Mullon

Claudy Jean Paul Mullon

Henry Santangini

A cell culture unit is disclosed herewith comprising a shell having two ends with an elongated chamber there between. A liquid and a gas perfusion port are located on each end. Liquid perfusion fibers are connected to the liquid perfusion ports and gas perfusion fibers are connected to the gas perfusion ports. These fibers thereby define an extracapillary and intracapillary space. The cell culture unit further comprises means for communicating with the intracapillary space of the gas perfusion fibers means for communicating with the intracapillary space of the liquid perfusion fibers, and means for communicating with the extracapillary space.

Dual fiber bioreactor

An artificial liver support system is described herein which comprises cryopreserved hepatocytes having an initial viability of 80-99%. Further disclosed are hepatocytes cryopreserved by dispensing hepatocytes into freezing containers, freezing the containers from between minus 50 to minus 90 degrees Celsius, storing the containers in liquid or vapor nitrogen, thawing the cryopreserved hepatocytes when ready for use and removing residual cryoprotectant media.

Method of cryopreserving hepatocytes

The invention features a virally-immortalized mammalian hepatocyte, which is derived from a normal liver cell, has differentiated hepatocyte-specific metabolic activity, has the ability to proliferate, and is nontumorigenic after prolonged culture.

Immortalized hepatocytes

A perfusion device such as a liver assist device containing a housing defining a perfusion inlet and a perfusion outlet, a porous membrane structure mounted within said housing to define a perfusion compartment and an adjacent hepatocyte compartment, and porcine hepatocytes isolated from a porcine liver by retrograde perfusion.

Isolation and culture of porcine hepatocytes

An artificial liver support system is described herein which comprises cryopreserved hepatocytes having an initial viability of 80-99% and a metabolic activity 50-80% of fresh hepatocytes. Further disclosed are hepatocytes cryopreserved by dispensing hepatocytes into freezing containers, freezing the containers from between minus 50 to minus 90 degrees Celsius, storing the containers in liquid or vapor nitrogen, thawing the cryopreserved hepatocytes when ready for use and removing residual cryoprotectant media.

Cryopreserved hepatocytes and high viability and metabolic activity

A cell culture unit is disclosed herewith comprising a shell having two ends with an elongated chamber them between. A liquid and a gas perfusion port are located on each end. Liquid perfusion fibers are connected to the liquid perfusion ports and gas perfusion fibers are connected to the gas perfusion ports. These fibers thereby define an extracapillary and intracapillary space. The cell culture unit further comprises means for communicating with the intracapillary space of the gas perfusion fibers, means for communicating with the intracapillary space of the liquid perfusion fibers, and means for communicating with the extracapillary space.

A perfusion device to grow and maintain hepatocytes including a chamber having a perfusion inlet and a perfusion outlet, a semipermeable membrane in the chamber defining separate perfusion and hepatocyte compartments, and hepatocytes in the hepatocyte compartment attached via oligosaccharide-lectin recognition linkage to a biopolymer support in the hepatocyte compartment.

Perfusion device with hepatocytes

An implantable prosthetic device made of biocompatible polymer and having a substantially continuous layer of autologus living cells attached via oligosaccharidelectin recognition linkages.

Growing community of inventors

Providence, RI, United States of America

Hugo O Jauregui