J. T. Baker Chemical Company

J. T. Baker, Inc.

Mallinckrodt Baker, Inc.

Hugh E Ramsden

Joseph M Patterson, Iii

David R Nau

Laura J Crane

Sunil V Kakodkar

Michael Henry

Joseph M Patterson

Dawn L Ward

Quaternized non-crosslinked polyethyleneimine silica based solid supports are useful strong anion exchangers for column chromatography separation and purification of proteins and have high binding capacity at high pH, the ability to bind basic proteins having high pH isoelectric points and are rapidly equilibrated and are essentially non-titratable.

Quaternized PEI silica solid supports for chromatography

Sulfonic derivatives of N-acylated covalently bound, non-crosslinked polyethyleneimine bonded phase silicas of the formula: ##STR1## wherein ##STR2## is the backbone of a silica gel or glass, n is an integer such that the polyethyleneiminopropyl silane group has an average molecular weight of from about 400 to about 1800; q is an integer of zero or one; R is selected from the group consisting of hydrogen, --CH.sub.2).sub.x H or --CH.sub.2).sub.m COOH where X is an integer of 1 or 2 and m is an integer of zero or 1; when R is hydrogen or --CH.sub.2).sub.x H then R.sup.1 is --CH.sub.2).sub.p COOH where p is an integer of zero or 1, and when R is --CH.sub.2).sub.m COOH then R.sup.1 is selected from the group consisting of hydrogen or --CH.sub.2).sub.y H where y is an integer of zero or 1, are especially useful as solid phases for the purification and separation of proteins having isoelectric points of below about 5.

N-acylated derivatives of polyethyleneimine bonded phase silica products

Sulfonic derivatives of N-acylated covalently bound, non-crosslinked polyethyleneimine bonded phase silicas of the formula: ##STR1## wherein is the backbone of a silica gel or glass, n is an integer such that the polyethyleneiminopropyl silane group has an average molecular weight of from about 400 to about 1800; q is an integer of zero or one; R is selected from the group consisting of hydrogen, --CH.sub.2).sub.x H or --CH.sub.2).sub.m COOH where x is an integer of 1 or 2 and m is an integer of zero or 1; when R is hydrogen or --CH.sub.2).sub.x H then R.sup.1 is --CH.sub.2).sub.p COOH where p is an integer of zero or 1, and when R is --CH.sub.2).sub.m COOH then R.sup.1 is selected from the group consisting of hydrogen or --CH.sub.2).sub.y H where y is a integer of zero or 1, are especially useful as solid phases for the purification and separation of proteins having isoelectric points of below about 5.

Use of sulfonic derivatives of acylated polyethyleneimine bonded phase

The reaction product of silica gel or controlled pore glass and carboalkoxyalkyl silanes are suitable for use as packing in solid phase extraction columns for cleanup of urine samples for analysis of cannabinoids.

Bonded phase of silica and carboalkoxyalkyl silanes for solid phase

A bonded phase silica product of the formula: ##STR1## in which ##STR2## is the backbone of a silica gel or controlled pore glass, X is selected from --O--, --S--, ##STR3## each R and R.sup.1 are each independently selected from hydrogen, an alkyl group of from 1 to 3 carbon atoms and --CH.sub.2).sub.m COOR.sup.3, R.sup.2 and R.sup.3 are each independently alkyl of from 1 to 4 carbon atoms, n is an integer of from 2 to 5, p is zero or one and m is an interger of from 1 to 4, is suitable for use in solid phase extraction for cleanup of urine samples for analysis of cannabinoids.

Bonded phase of silica for solid phase extraction

Sulfonic derivatives of N-acylated covalently bound, non-crosslinked polyethyleneimine bonded phase silicas of the formula: ##STR1## is the backbone of a silica gel or controlled pore glass, n is an integer such that the polyethyleneiminopropyl silane group has an average molecular weight of from about 400 to about 1800; q is an integer of zero or one; R is selected from the group consisting of hydrogen, --(CH.sub.2).sub.x H or --(CH.sub.2).sub.m COOH where x is an integer of 1 or 2 and m is an integer of zero or 1; when R is hydrogen or --(CH.sub.2).sub.x H then R.sup.1 is --(CH.sub.2).sub.p COOH where p is an integer of zero or 1, and when R is --(CH.sub.2).sub.m COOH then R.sup.1 is selected from the group consisting of hydrogen or --(CH.sub.2).sub.y H where y is an integer of zero or 1, are especially useful as solid phases for the purification and separation of proteins having isoelectric points of below about 5.

Sulfonic derivatives of acylated polyethyleneimine bonded phase silica

A bonded phase silica product of the formula: ##STR1## in which ##STR2## is the backbone of a silica gel or controlled pore glass, X is selected from --O--, --S--, ##STR3## each R and R.sup.1 are each independently selected from hydrogen, an alkyl group of from 1 to 3 carbon atoms and --CH.sub.2).sub.m COOR.sup.3, R.sup.2 and R.sup.3 are each independently alkyl of from 1 to 4 carbon atoms, n is an integer of from 2 to 5, p is zero or one and m is an integer of from 1 to 4, is suitable for use in solid phase extraction for cleanup of urine samples for analysis of cannabinoids.

The acylated reaction product of silica gel or controlled pore glass and polyethyleniminopropyl trimethoxy silane suitable for use as chromatographic column packing, wherein said acyl function does not have a terminal carboxyl.

Acylated polyethylenimine bound chromatographic packing

The reaction product of silica gel or controlled pore glass and polyethyleniminopropyl trimethoxy silane suitable for use as chromatographic column packing.

Polyethylenimine bound chromatographic packing

p-Nitrophenylester of succinoylaminopropyl silica gel is useful as an affinity matrix for reacting with ligand for use in affinity chromatography. The affinity matrix, upon reaction with a ligand, releases one molecule of p-nitrophenolate ion for every molecule of ligand that reacts and the solution turns yellow as the reaction occurs.

Affinity chromatography matrix with built-in reaction indicator

Improved diazonium affinity matrixes are provided which are storable stable and may be stored for extended periods for future use. The improved affinity matrixes are diazonium silica gel affinity matrixes containing an amine linkage and especially the fluoborate salts thereof.

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Scotch Plains, NJ, United States of America

Hugh E Ramsden