Howard Florey Institute of Experimental Physiology and Medicine

Hugh D Niall

Geoffrey W Tregear

Peter J Hudson

John Shine

Jennifer D Penschow

John P Coghlan

Yu-Cang Du

A method for determining the presence and location in animal or plant tissue of a specific polynucleotide population comprising: (a) preparing a section of the tissue to be examined; (b) contacting the tissue section under hybridization conditions with a synthetic, labelled oligonucleotide probe which is complementary to a representative portion of the target polynucleotide; (c) removing unhybridized probe material from the tissue section; and (d) detecting or identifying the locations in the tissue section where labelling or hybridization of the labelled probe has occurred. A diagnostic kit for use in performance of the above method is also disclosed.

Hybridization histochemistry method for determining the presence and location in animal or plant tissue of RNA

A method for determining the presence and location in animal or plant tissue of a specific polynucleotide population comprising: (a) preparing a section of the tissue to be examined; (b) contacting the tissue section under hybridization conditions with a synthetic, labelled oligonucleotide probe which is complementary to a representative portion of the target polynucleotide; (c) removing unhybridized probe material from the tissue section; and (d) detecting or identifying the locations in the tissue section where labelling by hybridization of the labelled probe has occurred. A diagnostic kit for use in performance of the above method is also disclosed.

Hybridization histochemistry method for determining the presence and

Genes and DNA transfer vectors for the expression of human preprorelaxin; sub-units thereof, including genes and transfer vectors for expression of human prorelaxin and the individual A, B and C peptide chains thereof; and equivalents of all such genes. Methods for synthesis of the peptides involving recombinant DNA techniques.

Process for synthesizing human H2-prorelaxin, human H2-relaxin and

Genes and DNA transfer vector for the expression of human prepororelaxin; subunits thereof, including genes and transfer vectors for expression of human profelaxin and the individual A, B and C peptide chains thereof; and equivalents of al such genes. Methods for synthesis of the peptides involving recombinant DNA techniques.

Process for synthesizing human H1-prorelaxin, H1-relaxin and fusion

Human relaxin polypeptides

Genes and DNA transfer vector for the expression of human preprorelaxin; subunits thereof, including genes and transfer vectors for expression of human prorelaxin and the individual A, B and C peptide chains thereof; and equivalents of all such genes. Methods for synthesis of the peptides involving recombinant DNA techniques.

Human pro relaxin polypeptides

Genes and DNA transfer vectors for the expression of human preprorelaxin; sub-units thereof, including genes and transfer vectors for expression of human prorelaxin and the individual A, B and C peptide chains thereof; and equivalents of all such genes. Method for synthesis of the peptides involving recombinant DNA techniques are provided.

Molecular cloning and characterization of a gene sequence coding for

Molecular cloning and characterization of a further gene sequence coding

A method for the synthesis of porcine relaxin or modified forms or analogues thereof, which comprises the steps of reducing a mixed solution of the S-sulfonated A and B peptide chains, or modified forms or analogues thereof; precipitating the reduced peptides with acetone; washing the mixed peptides; oxidizing the mixed peptides in the presence of aqueous sodium chloride; and isolating the relaxin thus produced. Also claimed are modified forms and analogues of porcine relaxin analogues which comprise shortened forms of the natural B and/or A peptide chains, and/or in which one or both of A and B chains in the full length or shortened form has been chemically modified.

Growing community of inventors

Elwood, Australia

Hugh D Niall