Eastman-Kodak Company

Hugh Arthur Risley

Charles T Goodhue

Roy Eugene Snoke

Gary M Underwood

A stable uricase preparation of high activity is produced by growing Micrococcus luteus NRRL B-8166 in a nutrient medium. Uricase preparations having activities of up to about 1000 U/liter are produced.

Production of uricase from micrococcus luteus

An integral analytical element for analysis of liquids for their cholesterol content is disclosed. The element is of the type which comprises at least two superposed layers including a spreading layer and a reagent layer in fluid contact and, optionally, a support. Cholesterol oxidase and a composition for the hydrolysis of cholesterol esters comprising lipase having cholesterol esterase activity and protease are included in the element such that cholesterol esters contained in a sample applied to the spreading layer are saponified to free cholesterol and free cholesterol is decomposed in the presence of cholesterol oxidase to produce a detectable change related to the total cholesterol content of the sample.

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Newport Richey, FL, United States of America

Hugh Arthur Risley