Perstorp Ab

Rijksuniversiteit Te Leiden

Hubertus Irth

Lars Persson

Ruth Kornfeldt

Eva Saskia Lutz

Jan Van Der Greef

Aaike Johannes Oosterkamp

The present invention relates to an on-line detection method comprising the on-line coupling of a fractionation and a biochemical detection technique, which method comprises the addition of a controlled amount of an affinity molecule to the effluent of the fractionation step to react with analytes in the effluent, the subsequent addition of a controlled amount of a detectable ligand capable of binding to the affinity molecule, and detection of the affinity molecule/detectable ligand complex. Further, the invention is directed to an on-line method for the screening of compounds on their binding capability to a known affinity molecule, which method comprises a fractionation step providing an effluent, the addition of a controlled amount of said affinity molecule to the effluent of the fractionation step and effecting a contact time sufficient to allow a reaction with or interaction between the compounds in the effluent, the subsequent addition of a controlled amount of a detectable ligand capable of binding to the affinity molecule, and detection of the affinity molecule/detectable ligand complex.

On-line coupling biochemical detection to continuous flow separation

Method for analytical separation of inositol phosphates with quaternary

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Amsterdam, Netherlands

Hubertus Irth