Kali-chemie Aktiengesellschaft

Hubert Schindler

Guenter Weidenbach

Dirk Bonse

Hans-Joerg Mueller

Winfried Kuhrt

Hermann Fischer

Enzymes are immboilized on an inorganic carrier which is a round, macropermeable agglomerate having a mulberry-type structure formed from spherical microporous .alpha.-aluminum oxide hydroxide particles. An organic polycondensate having free organic functional groups as enzyme binding sites penetrates the agglomerate. The .alpha.-aluminum oxide hydroxide particles of the agglomerate retain essentially their form prior to agglomeration. The polycondensate is prepared by reacting a polyimine with a dialdehyde or an epoxy compound. Preferably, the polyimine is polyethyleneimine, the dialdehyde is glutardialdehyde and the epoxy compound is an epoxy-functional silicon resin, an aliphatic diepoxide or an aliphatic triepoxide. The agglomerate is prepared by granulating the .alpha.-aluminum oxide hydroxide particles in the presence of the polyimine to form the agglomerate having the polyimine dispersed throughout and then reacting the polyimine with the dialdehyde or the epoxy compound. A preferred enzyme is glucose isomerase.

Enzyme immobilzation on a macropermable agglomerate of microporous .alpha.-

Disclosed is process for the preparation of alumina-containing particles, comprising the steps of (a) mixing an aluminum oxide hydrosol containing a hydrate of alumina or activated alumina and an additive component selected from a high molecular weight natural organic material which is insoluble in aqueous solution up to a temperature of 50.degree. C. and carbon with a solution of hydrolyzable base to form a mixture; (b) introducing the mixture in dispersed form into a water-immiscible liquid having a temperature of from about 60 to 100.degree. C., whereby gel particles are formed; (c) aging the gel particles in the liquid at the temperature and subsequently in an aqueous ammonia solution; (d) recovering the aged particles; and (e) calcining the recovered particles.

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Uetze-Dollbergen, Germany

Hubert Schindler