Hoechst Aktiengesellschaft

Hubert Mullner

Gunter Donn

Eugen Uhlmann

Wolfgang Konig

Alfred Puhler

Wolfgang Wohlleben

Klaus Bartsch

Rudolf Schneider

Doris Hillemann

Inge Broer

Rainer Obermeier

Rolf Geiger

Peter Eckes

Bernadus Uijtewaal

Bernhard Kutscher

Horst Kessler

Paul Habermann

Joachim Engels

Waldemar Wetekam

Jan Glauder

A multifunctional RNA having self-processing activity, the preparation thereof and the use thereof Host cells can be transformed so that they express ribozyme RNA and antisense RNA which are connected with each other via a spacer. The RNA molecules can, for example, be complementary to a certain viral RNA. Plants which have been transformed with genes coding for RNA of this type show a significantly improved resistance to viruses.

Multifunctional RNA having self-processing activity, the preparation

Virus genes, such as coat protein genes, which confer viral resistance or bring about a reduction in the signs of infection by the corresponding virus can be combined with herbicide-resistance genes, such as the phosphinothricin resistance gene, for the transformation of plants. A combination of a viral gene and a herbicide-resistance gene facilitates the selection of transgenic plants. In addition, in practical field cultivation, the vitality of the plants is increased by the virus tolerance, and an improved plant protection is possible due to the herbicide resistance gene.

Virus/herbicide resistance genes, processes for producing same and their

Deacetylase genes for the production of phosphinothricin or phosphinothricyl-alanyl-alanine, processes for their isolation, and their use. The invention relates to deacetylase genes, to processes for their isolation, and to their use, in particular for the production of transgenic plants using tissue-specific promoters. It is possible to prevent the development of certain parts in these plants in a targeted manner. With the aid of deacetylase genes, it is furthermore possible to identify and isolate tissue-specific promoters in transgenic plants.

Deacetylase genes for the production of phosphinothricin or

A multifunctional RNA having self-processing activity, the preparation thereof and the use thereof. Host cells can be transformed so that they express ribozyme RNA and antisense RNA which are connected with each other via a spacer. The RNA molecules can, for example, be complementary to a certain viral RNA. Plants which have been transformed with genes coding for RNA of this type show a significantly improved resistance to viruses.

The invention relates to deacetylase genes, to processes for their isolation, and to their use, in particular for the production of transgenic plants using tissue-specific promoters. It is possible to prevent the development of certain parts in these plants in a targeted manner. With the aid of deacetylase genes, it is furthermore possible to identify and isolate tissue-specific promoters in transgenic plants.

Expression of a multigene RNA having self-splicing activity An RNA is expressed which can release two or more active genes by means of inserted ribozyme structures. The expression can be carried out in plants or other organisms.

Expression of a multigene RNA having self-splicing activity

Polypeptides having a carboxamide carboxyl terminal end and a methionine residue, which is optionally bonded to a bacterial protein, on the amino end can be prepared by synthesis by genetic engineering methods of the corresponding polypeptide having at the C terminal end a glycine residue, and conversion of the latter enzymatically into the amino group of the desired carboxamide group. Peptides which have the aminoacid sequence of growth hormone releasing factor, part sequences thereof, or modifications of these peptides, are readily accessible by this means. The synthesis by genetic engineering methods is advantageously carried out via two gene fragments which are synthesized chemically from smaller, single-stranded structural units. The two gene fragments are then linked enzymatically to give the complete gene, which is incorporated into a suitable vector, amplified there, and the peptide is isolated directly or as a fused protein, and is converted enzymatically into the desired amide.

Preparation of polypeptides having an amide carboxyl terminal end

Expression of a gene coding for human granulocyte macrophage colony-stimulating factor (CSF) in bacteria results in CSF proteins which are biologically active. Modification of the natural or of a synthetic gene structure results in biologically active derivatives with a modified amino acid sequence.

GM-CSF protein, its derivatives, the preparation of proteins of this

A chromophoric peptide is prepared of the formula

Chromophoric peptides for detecting peptidylglycine-.alpha.-amidizing

The invention relates to a process for the preparation of peptides of the general formula

Process for the preparation of pentapeptides having an action on the

The invention relates to cyclopeptides of the formula I ##STR1## in which A denotes Lys, D-Lys, Arg or D-Arg, B denotes Lys, D-Lys, Arg or D-Arg, C denotes Asp, D-Asp, Glu or D-Glu, U denotes Val or D-Val and D denotes Tyr, D-Tyr, Trp, D-Trp, Phe or D-Phe, 1 to 4 of the radicals A, B, C, U and D being in the L-configuration and the remaining radicals being in the D-configuration, processes for their preparation, their use and peptidic precursors and processes for their preparation.

Novel peptides having an immunostimulating action, processes for their

The invention provides a nonapeptide of the formula I

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Kelkheim, Germany

Hubert Mullner