Millipore Corporation

Perseptive Biosystems Inc.

Hubert Koester

James M Coull

Brian D Gildea

Michael J Steuck

Alex Garfield Bonner

Malcolm G Pluskal

Darryl J Pappin

Darryl J Rappin

The invention pertains to compounds and methods for the reversible modification of natural products, biopolymers or synthons for natural products or biopolymers, such as nucleosides, nucleotides, and oligonucleosides. The modification enables one to perform a variety of chemistries on these compounds, yet can be removed to regenerate functional groups on the natural products, biopolymer or synthon of interest.

Succinimidyl trityl compounds and a process for preparing same

The invention pertains to a method for immobilizing proteins or peptides onto a flat, microporous membrane surface in a form suitable for sequence analysis or other chemical or enzymatic processes. The process involves the formation of a thin polymer network that entraps the protein or peptide therein.

Immobilization of proteins and peptides on insoluble supports

A membrane suitable for immobilizing peptides and proteins is disclosed. The membrane is a flexible, polymeric, porous membrane which contains functional groups capable of covalently linking the peptides and proteins. The functional groups can be provided by reacting the membrane itself or a coating thereon with nucleophiles which provide --NH.sub.2, --SH, --OH or --COOH functionality to the membrane surface. Additionally, surfaces containing --NH.sub.2 groups can be further reacted with diisothiocyantes to provide an isothiocyanate functionality having enhanced covalent binding characteristics.

Membranes for solid phase protein sequencing

A method is provided for synthesizing oligonucleotides and peptides directly onto a membrane. The method provides a means for generating membrane affinity supports. A modified membrane for the method of direct synthesis is also provided.

Growing community of inventors

Concord, MA, United States of America

Hubert Koester