Ses Europe N.v./s.a.

Sesvanderhave Nv

Hubert Guilley

Ken Richards

Emmanuelle Lauber

Gerard Jonard

Cornelis Maria Petrus Van Dun

Guy Weyens

Marc Lefebvre

Elodie Klein

David Gilmer

Kenneth Richards

Gérard Jonard

G&eacute;rard Jonard

Claudine Bleykasten-Grosshans

Salah Bouzoubaa

The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence. The invention further relates to genetically modified TGB-3 viral sequences suitable to induce gene silencing. In particular hairpin constructs based on such sequences proved highly efficient to induce a PTGS mechanism and degradation of the whole of RNA2 thereby. When plants are transformed accordingly the spread of the virus in the plant is significantly reduced or blocked.

P15 hairpin constructs and use

The present invention relates to a method for conveying resistance to beet necrotic yellow vein virus (BNYVV) to a sugar beet plant, which method comprises the following steps: (a) preparing a DNA fragment consisting of a nucleotide sequence that corresponds to nucleotides 153 to 3258 of the genomic RNA 1 of the beet necrotic yellow vein virus (BNYVV); (b) introducing said DNA fragment, operatively linked to a promoter that is active in sugar beet plants, into a sugar beet plant cell to obtain a transformed sugar beet cell; and (c) regenerating a transgenic sugar beet plant from the transformed sugar beet plant cell.

Method of conveying BNYVV resistance to sugar beet plants

The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by the TGB-3 viral sequence, comprising the following successive steps: submitting the sequence to point mutation(s) which allow the substitution of at least one amino-acid into a different amino-acid, selecting genetically modified TGB-3 wild type viral sequences having the point mutation(s) and which are not able to promote cell-to-cell movement of a mutant virus having a dysfunctional TGB-3 wild type viral sequence, when expressed in trans from a replicon, further selecting among the genetically modified TGB-3 viral sequences, the specifically genetically modified sequence which inhibits infection with a co-inoculated wild type virus when the mutant form was expressed from a replicon, and recovering the specifically genetically modified TGB-3 viral sequence.

Method of genetic modification of a wild type viral sequence

The present invention concerns a method for inducing resistance to a virus comprising a TGB3 sequence with the proviso that it is not the potato virus X, into a plant cell or plant, comprising the following steps: preparing a nucleic acid construct comprising a nucleic acid sequence corresponding to at least 70% of the nucleic acid sequence of TGB3 of said virus or its corresponding cDNA, being operably linked to one or more regulatory sequence(s) active in a plant, transforming a plant cell with the nucleic acid construct, and possibly regenerating a transgenic plant from the transformed plant cell. The present invention is also related to the plant obtained.

Method for inducing viral resistance into a plant

Growing community of inventors

Berstett, France

Hubert Guilley