Pioneer Hi-bred International, Inc.

Huaxun Ye

William James Gordon-Kamm

Keith S Lowe

Jon Aaron Tucker Reinders

Tim Fox

Leandro Daniel Perugini

Rachel Carol Huegel

Michael T Elsing

The methods disclosed herein provide for clonal plant production using a morphogenic gene to produce non-transgenic and transgenic plants wherein the morphogenic gene does not integrate into the genome of the plant.

Methods for clonal plant production

Compositions and methods are provided for the use of pollen-inhibitor genes and/or color marker genes in accelerated trait introgression. Compositions and methods are also provided for introducing a pollen-inhibitor gene and/or a color marker gene in close proximity to a trait locus of interest. Breeding methods and methods for selecting plants comprising a trait locus of interest in close proximity to at least one pollen-inhibitor gene and/or color marker gene are also disclosed. The methods and compositions employ at least one pollen-inhibitor gene and/or color marker gene to provide an effective system for accelerated trait introgression in the genome of a plant.

Methods and compositions for accelerated trait introgression

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

Systems and methods for cellular reprogramming of a plant cell

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Johnston, IA, United States of America

Huaxun Ye