Nanjing Tech University

Huanqing Niu

Yong Chen

Dong Liu

Hanjie Ying

Pingkai Ouyang

Qingguo Liu

Ming Lei

Bin Yu

Ming Li

Di Zhang

Wei Zhuang

Chenjie Zhu

Zhuotao Tan

Pengpeng Yang

Xingyuan Cao

Yaoying Han

Yaping Fu

Xiwei Peng

Shishi Wu

Yuhan Yang

A method for preparing a nitrogen-containing heterocyclic compound and a derivative thereof by an enzymatic-chemical cascade method, comprising: reacting an alcohol, an amine, an alcohol dehydrogenase, a flavin molecule and a coenzyme in a solvent to obtain the nitrogen-containing heterocyclic compound and the derivative thereof; compared with the prior art, the method is a green and economical enzymatic-chemical cascade method, and is used for synthesizing nitrogen-containing heterocyclic compounds and derivatives thereof; compared with a common toxic chemical catalyst, the alcohol dehydrogenase is selected as a catalyst in the method, which has the characteristics of high substrate specificity, no pollution, high catalytic efficiency, no toxic solvents and simple post-treatment.

Method for preparing nitrogen-containing heterocyclic compound and derivative thereof by enzymatic-chemical cascade method

The invention discloses a recombinantfor producing lysine by biofilm continuous fermentation and a construction method thereof, wherein the recombinantis constructed by overexpressing a protease gene FtsH in a; and the construction method comprises the following steps of: (1) performing PCR on a genome of the, and amplifying the FtsH gene to obtain an amplified FtsH gene segment; (2) cloning the FtsH gene segment to an overexpression plasmid to obtain a recombinant plasmid; and (3) introducing the recombinant plasmid into the, and screening to obtain the recombinant. The invention has the beneficial effects that thefor overexpressing the protease FtsH is constructed in the invention, and a film-forming ability of theis enhanced, such that a yield of continuous immobilized fermentation of theis increased by 38.2% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 26.4%.

Recombinant corynebacterium glutamicum for producing lysine by biofilm continuous fermentation and construction method thereof

The invention discloses a method for producing lysine by utilizing adsorption and immobilized fermentation of a recombinant, wherein the recombinantis constructed by simultaneously overexpressing an adenosine triphosphate ATPase while knocking out an extracellular nuclease ExeR in a. The recombinantcan effectively improve eDNA secretion of theand reduce eDNA degradation of the, so that thecan be more easily adsorbed on a surface of a solid carrier for immobilized fermentation, such that a yield of continuous immobilized fermentation of theis increased by 49.67% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 29.17%.

Method for producing lysine by utilizing adsorption and immobilized fermentation of recombinant corynebacterium glutamicum

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Jiangsu, China

Huanqing Niu