Mgi Tech Co., Ltd.

Huanhuan Liu

Wenwei Zhang

Chongjun Xu

Yue Zheng

Yuliang Dong

Zhougang Zhang

Yujun Zhou

Xing Liu

Xi Zhang

Snezana Drmanac

Provided are a group of phi29 DNA polymerase mutants having increased thermal stability and use thereof. The phi29 DNA polymerase mutants are proteins obtained by performing point mutation A and/or point mutation B and/or point mutation C on phi29 DNA polymerase, the point mutation A meaning that an amino acid residue M at position 97 of the phi29 DNA polymerase is mutated to other amino acid residue, the point mutation B meaning that an amino acid residue L at position 123 of the phi29 DNA polymerase is mutated into other amino acid residue, and the point mutation C meaning that an amino acid residue E at position 515 of the phi29 DNA polymerase is mutated to other amino acid residue. The stability of the phi29 DNA polymerase mutants is higher than that of a wild-type phi29 DNA polymerase.

Phi29 DNA polymerase mutant having increased thermal stability and use thereof

Provided are a phi29 DNA polymerase mutant with increased thermo stability, a method for preparing the mutant, the use of the mutant, and a method for increasing the stability of the phi29 DNA polymerase.

PHi29 DNA polymerase mutant with increased thermostability

Growing community of inventors

Shenzhen, China

Huanhuan Liu