Mgi Tech Co., Ltd.

Bgi Genomics Co., Limited

Huaiqian Xu

Jun Wang

Radoje Drmanac

Jian Wang

Hui Fu Jiang

Wenwei Zhang

Xiao Liu

Yuan Jiang

Chunyu Geng

Xia Zhao

Jing Guo

Huanming Yang

Kai Tian

Xiaojun Ji

Junjie Zhang

Ruifang Zhang

Hui Jiang

Provided are a vesicular adaptor and a single-chain cyclic library constructed by using the adaptor. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantage of high throughput sequencing, high accuracy and simple operations.

Vesicular adaptor and uses thereof in nucleic acid library construction and sequencing

Provided are a vesicular linker and a single-chain cyclic library constructed by using the linker. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantages of high throughput sequencing, high accuracy and simple operations.

Vesicular linker and uses thereof in nucleic acid library construction and sequencing

Provided is a method of constructing a sequencing library using an adaptor element in a bubble shape. The bubble-shaped adaptor is ligated to a DNA fragment respectively at the 3'-terminal and the 5&#x2032;-terminal i.e., two bubble-shaped adaptors with same sequences are ligated in one step. The bubble-shaped adaptor-ligated product is then amplified with a primer complementary to the 3&#x2032;-terminal of the long-chain nucleic acid of the bubble-shaped adaptor, so as to replace the non-paired sequence in the short-chain nucleic acid.

Method of constructing sequencing library with bubble-shaped adaptor element

Growing community of inventors

Shenzhen, China

Huaiqian Xu