Hangzhou Calibra Diagnostics Co., Ltd.

Hangzhou Dian Medical Inspection Center Co., Ltd.

Hangzhou Calibra Technologies Co., Ltd.

Huafen Liu

Qi Zhang

Ziqing Kong

Pengyun Liu

Baoyu Han

Shanshan Zeng

Chao Zhang

Sili Wu

Xiaofen Yuan

Rongchang Chen

Yuning Zhu

Yuan Wang

Yikun Li

Yabo Wang

Shishan Fu

Jinfei Ma

Weijia Wu

Jianbing Du

The present invention describes a test kit for the detection of fat-soluble vitamins in serum using a method based on high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). The internal standard (IS) solution included in the kit is based on methanol, acetonitrile and isopropyl alcohol solvents. Ammonium acetate is added to the IS solution to make it more stable and last for longer storage time. The application of this kit can significantly improve the recovery and detection sensitivity of vitamins A, E, K1 and K2 in serum without additional sample enrichment, make sample preparation process simpler and more efficient, keep processed samples stable for longer time, and lower the overall cost for more accurate and repeatable test results.

Test kit for testing fat-soluble vitamins in serum using high performance liquid chromatography coupled with tandem mass spectrometry

A detection kit for detecting an immunosuppressor in whole blood by high performance liquid chromatography-tandem mass spectrometry and a detection method thereof is provided. An internal standard solution is added with an antioxidant, vitamin E, and mixed with an internal standard diluent containing zinc sulfate heptahydrate, purified water and methanol for sample pretreatment, which not only exerts the function of the internal standard, but also synchronously achieves erythrocyte treatment, protein precipitation and target substance extraction. Various embodiments enable the immunosuppressor to be more stable in a solution matrix, thus promoting the detection accuracy and sensitivity. Various embodiments adopt isotopically-labeled sirolimus as an internal standard of everolimus to substitute isotopically-labeled everolimus, thus overcoming the interference of everolimus on isotopically-labeled everolimus and satisfying the detection requirements. Various embodiments detect four immunosuppressors simultaneously to reduce the cost of the internal standard, and has a lower detection cost, more accurate and stable detection results.

Detection kit for detecting immunosuppressors in whole blood by high performance liquid chromatography-tandem mass spectrometry and detection method thereof

A full-automatic sample pretreatment device includes a frame module, a cap removing module, a blood-collection tube grasping module, a pipette module, a platform module, a handling module, a film sealing module, a magnetic solid phase extraction module, an SPE positive pressure extraction module and a conveyor belt waste transfer module. A conveyor belt waste transfer module is arranged at a bottom of the frame module, the cap removing module, the platform module and the magnetic solid phase extraction module are sequentially arranged in the middle of the frame module from left to right, and the blood-collection tube grasping module, the pipette module, the handling module and the film sealing module are arranged at an upper portion of the frame module. The magnetic solid phase extraction module and the SPE positive pressure extraction module are detachable and interchangeable components.

Device for sample pretreatment

The present invention relates to a system for predicting gestational diabetes mellitus (GDM) of pregnant individuals, wherein the system comprises an operation module, and the operation model comprises a support vector regression model, and the system is used to predict the plasma glucose levels at 1 hour and/or 2 hours of oral glucose tolerance test (OGTT) by using a support vector regression developed prediction model generated by substituting the concentration of the biomarkers in fasting blood samples of pregnant individuals. The present invention provides biomarkers and biomarker-based diagnostic models for differential diagnosis of gestational diabetes mellitus (GDM), which can be applied to diagnosis or prediction of GDM in early stage and are of great significance to the prevention or treatment of GDM.

Method and system for rapid prediction offast blood glucose level in pregnant subjects

In a screening kit and a confirmed and typing diagnosis system for primary aldosteronism, a sample is pretreated by a magnetic bead bonded with a balanced hydrophilic-lipophilic polymer on the surface thereof, and process conditions are optimized and the content of each the five markers such as, aldosterone in the sample is accurately detected by liquid chromatography-tandem mass spectrometry for one time, thus finding the optimal screening cut-off value of 20.4; when a positive result is judged, PA is confirmed and subjected to typing diagnosis according to the test values of the markers, thereby achieving the simultaneous detection of the content of each the five markers such as, aldosterone on the same platform. Therefore, the screening kit and confirmed and typing diagnosis system for primary aldosteronism are integrated with screening, confirmed and typing diagnosis functions, thus providing a reliable laboratory examination basis for clinicians to formulate an effective therapeutic regimen.

Screening kit and diagnosis system for primary aldosteronism

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Hangzhou, China

Huafen Liu