National Health Research Institutes

Academia Sinica

National Cheng-Kung University

Hsin-Wei Chen

Chih-Hsiang Leng

Pele Choi-Sing Chong

Shih-Jen Liu

Ming-Hsi Huang

Chi-Ling Tseng

Hsueh-Hung Liu

Dar-Bin Shieh

Steve R Roffler

Yung-Chun Chuang

Yee-Shin Lin

Trai-Ming Yeh

Chiung-Yi Huang

Shu-Ching Hsu

Shu-Ying Wang

Tzong-Shiann Ho

Chia-Yi Yu

Shu-Wen Wan

Yan Chak Kwok

Li-Tzu Wang

Hsin-Yu Chen

Peie Choi-Sing Chong

The present invention relates to a composition of subunit dengue vaccine comprising a fusion protein of conjugating or connecting delta C nonstructural protein 1 (NS1&#x394;C or truncated NS1&#x394;C) to at least one polypeptides of NS3c (or truncated NS3c) and/or consensus envelope protein domain III (cEDIII), thereby enhancing better protection against DENV challenge and alleviating associated pathological effects.

Composition of subunit dengue vaccine

Provided is a pharmaceutical composition, including an antigen fusion protein which includes an antigen and an antagonist of an Fc gamma receptor. Also provided is a method of enhancing immunogenicity of an antigen, including conjugating the antigen with an antagonist of an Fc gamma receptor to form an antigen fusion protein. Also provided is a method of enhancing an immune response to an antigen in a subject, including administering to the subject an effective amount of an antigen fusion protein which includes an antigen and an antagonist of an Fc gamma receptor. The present invention may be applied in the development of potent vaccines based on targeting vaccine antigens to antigen-presenting cells via binding to Fc gamma receptors.

Compositions and methods for enhancement of immune responses

Described herein are an immunogenic peptide containing the sequence of CLDSLGQWN (SEQ ID NO:2) and a method of using the peptide for treating cancer.

Immunogenic peptide containing a b cell epitope of tumor associated antigen L6

A composition in a water-in-oil-in-water (W/O/W) emulsion is disclosed. The composition comprises: (a) a continuous aqueous phase, comprising HO; (b) an oil phase or an oil shell, dispersed in the continuous aqueous phase; and (c) a hydrophilic polymer, stabilizing an interface between the continuous aqueous phase and the oil phase or the oil shell to form the water-in-oil-in-water (W/O/W) emulsion. The oil phase or the oil shell comprises: (i) oil; (ii) an internal aqueous phase, dispersed within the oil or the oil shell; and (iii) a lipophilic sorbitan-polyester conjugate, stabilizing an interface between the oil and the inner aqueous phase to form a water-in-oil (W/O) emulsion. The lipophilic sorbitan-polyester conjugate comprises: (1) sorbitan; and (2) poly(lactide-co-&#x3b5;-caprolactone) or polylactic acid (polylactide), conjugated to the sorbitan.

Sorbitan polyester conjugates for stabilizing water-in-oil emulsions and delivering controlled release of bioactive agents

This invention relates to an immunosuppressive cell, and methods of obtaining the cell and using the cell. The immunosuppressive cell is obtained by culturing a precursor cell in a medium that contains a GRO chemokine.

Immunosuppressive cells and methods of making and using thereof

A method of producing a recombinant lipidated polypeptide in. The method includes providing anhost cell adapted to express a recombinant lipidated polypeptide; and culturing thehost cell in a minimal medium under conditions that allow expression of the polypeptide in lipidated form.

Method of producing lipidated polypeptides

This invention relates to, inter alia, an isolated lipidated polypeptide including a lipid moiety at the N-terminus and a plurality of epitopes, and methods of making and using the polypeptide.

Lipidated polyepitope vaccines

Disclosed are polypeptides and fusion proteins. Also disclosed are related immunotherapeutic compositions and methods.

Lipidated tumor-associated antigens and immunotherapeutic compositions

Disclosed are lipopeptides or lipoproteins, related compositions, and related methods.

Adjuvants

An immunopeptide containing a T cell epitope from the tumor associated antigen L6 (TAL6) and a nucleic acid encoding the immunopeptide are described herein. The immunopeptide or its encoding nucleic acid can be used as a component of an immune composition for enhancing immune response against cancer cells that express TAL6.

Immunogenic peptides of tumor associated antigen L6 and uses thereof in cancer therapy

A method for enhancing a body's response to an immunogen is disclosed. The method comprises immunizing a subject in need thereof a vaccine composition in a water-in-oil (W/O/W) emulsion that comprises (a) an antigen; and (b) an adjuvant composition in a W/O/W emulsion. The adjuvant composition comprises: (i) a continuous aqueous phase comprising HO; (ii) an oil phase comprising oil dispersed in the continuous aqueous phase, comprising an internal aqueous phase comprising HO, being dispersed in the oil phase; and a physiologically acceptable lipophilic emulsifier selected from the group consisting of mannide monooleate and sorbitan esters, stabilizing the interface between the inner aqueous phase and the oil phase to form a water-in-oil (W/O) emulsion; and (iii) poly(ethylene glycol)-block-poly(lactide-co-&#x3b5;-caprolactone), stabilizing the interface between the oil phase and the continuous aqueous phase.

Multi-phase emulsions based on amphiphilic block copolymers

Production of a lipidated protein in anstrain adapted to membrane protein expression.

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Disclosed are immunogenic composition, fusion proteins, and related methods for inducing immune response to dengue virus infection.

Lipidated vaccine against dengue virus infection

A consensus peptide sequence designed for domain III of the envelope proteins of four serotypes of dengue virus and used in a vaccine against multiple serotypes of dengue virus is disclosed. The vaccine is able to elicit cross-neutralization antibody responses against multiple serotypes of dengue virus.

Consensus dengue virus envelope protein domain III polypeptides (cED III) and their methods of use

Production inof a lipidated fusion protein containing a lipidating sequence derived from Ag473 and a target polypeptide.

Growing community of inventors

Miaoli County, Taiwan

Hsin-Wei Chen