University of Kentucky Research Foundation

University of Texas System

Case Western Reserve University

Hsin-Hsiung Tai

Chang-Guo Zhan

Hoon Cho

Sanford D Markowitz

Joseph Ready

Stanton L Gerson

Amar Desai

Monika Antczak

Bruce Posner

Fang Zheng

Liu Xue

Melissa Moss

James K V Willson

Sung Yeun Yang

KiBeom Bae

Yongyou Zhang

Shurong Hou

Youngyou Zhang

Xiabin Chen

James KV Willson

Bruce A Posner

Kibeom Bae

The presently-disclosed subject matter includes isolated polypeptides that comprise a butyrylcholinestrase (BChE) polypeptide and a second polypeptide. The BChE polypeptide as well as the second polypeptide can be variants and/or fragments thereof. The presently-disclosed subject matter also includes a pharmaceutical composition that comprises the present isolated polypeptide and a suitable pharmaceutical carrier. Further still, methods are provided for treating cocaine-induced conditions, and comprise administering the isolated polypeptide and/or pharmaceutical compositions thereof to an individual.

Cocaine hydrolase-FC fusion proteins for cocaine and methods for utilizing the same

Compounds and methods of modulating 15-PGDH activity, modulating tissue prostaglandin levels, treating disease, diseases disorders, or conditions in which it is desired to modulate 15-PGDH activity and/or prostaglandin levels include 15-PGDH inhibitors and 15-PGDH activators described herein.

Compositions and methods of modulating 15-PGDH activity

A novel computational method and generation of mutant butyrylcholinesterase for cocaine hydrolysis is provided. The method includes molecular modeling a possible BChE mutant and conducting molecular dynamics simulations and hybrid quantum mechanical/molecular mechanical calculations thereby providing a screening method of possible BChE mutants by predicting which mutant will lead to a more stable transition state for a rate determining step. Site-directed mutagenesis, protein expression, and protein activity is conducted for mutants determined computationally as being good candidates for possible BChE mutants, i.e., ones predicted to have higher catalytic efficiency as compared with wild-type BChE. In addition, mutants A199S/A328W/Y332G, A199S/F227A/A328W/Y332G, A199S/S287G/A328W/Y332G, A199S/F227A/S287G/A328W/Y332G, and A199S/F227A/S287G/A328W/E441D all have enhanced catalytic efficiency for (&#x2212;)-cocaine compared with wild-type BChE.

High-activity mutants of butyrylcholinesterase for cocaine hydrolysis and method of generating the same

A formulation for use in detecting and/or determining peroxidase activity comprises a mixture in solution of tetramethylbenzidine, hydrogen peroxide, a buffering agent and bacitracin as a stabilizing agent. The formulation is used as a peroxidase substrate that is stable in solution for an extended period of time and provides enhanced color sensitivity.

Composition of, method of producing and method of using a stabilized

Growing community of inventors

Lexington, KY, United States of America

Hsin-Hsiung Tai