The University of Texas System

Los Alamos National Security, LLC

Hsin-Chih Yeh

Jennifer Suzanne Martinez

James Werner

Andrew Dunn

Yen-Liang Liu

Jaswinder Kumar Sharma

Yu-An Chen

Judy M Obliosca

Cong Liu

Evan Perillo

In one embodiment, compositions and methods are provided for the detection and/or quantification of epigenetic modifications in DNA. In particular aspects, probes are provided comprising fluorescent metal nanocluster beacons, which can selectively detect nucleic acids including an epigenetic modification.

Metal nanocluster beacons for detection of epigentic modifications

Systems and methods for particle tracking using spatiotemporal offset light beams. In exemplary embodiments, the optical systems and methods can be used with conventional two-photon microscopy equipment to perform high speed, high precision, and deep tissue three-dimensional single-particle tracking. Exemplary embodiments can be configured for single-molecule studies of biological diffusion and transport processes.

Systems and methods for particle tracking using spatiotemporal offset light beams

Described herein are nucleic acid based probes and methods for discriminating and detecting single nucleotide variants in nucleic acid molecules (e.g., DNA). The methods include use of a pair of probes can be used to detect and identify polymorphisms, for example single nucleotide polymorphism in DNA. The pair of probes emit a different fluorescent wavelength of light depending on the association and alignment of the probes when hybridized to a target nucleic acid molecule. Each pair of probes is capable of discriminating at least two different nucleic acid molecules that differ by at least a single nucleotide difference. The methods can probes can be used, for example, for detection of DNA polymorphisms that are indicative of a particular disease or condition.

Compositions and methods for detecting single nucleotide polymorphisms

A hybridization probe containing two linear strands of DNA lights up upon hybridization to a target DNA using silver nanoclusters that have been templated onto one of the DNA strands. Hybridization induces proximity between the nanoclusters on one strand and an overhang on the other strand, which results in enhanced fluorescence emission from the nanoclusters.

Probe and method for DNA detection

Growing community of inventors

Austin, TX, United States of America

Hsin-Chih Yeh