Whitehead Institute for Biomedical Research

Hsiang-Ying Lee

Harvey F Lodish

Hidde L Ploegh

Jiahai Shi

Lenka Hoffman

Novalia Pishesha

Xiaofei Gao

Methods for the in vitro production of enucleated red blood cells and the enucleated red blood cells thus prepared are provided. Such enucleated red blood cells may express a sortaggable surface protein, which allows for surface modification in the presence of a sortase. Also described herein are surface modified enucleated red blood cells, e.g., conjugated with an agent of interest such as a peptide, a detectable label, or a chemotherapeutic agent, and uses thereof in delivering the agent to a subject.

In vitro production of red blood cells with proteins comprising sortase recognition motifs

A population of early-stage burst-forming unit-eryhtoid (BFU-E) cells characterized by low expression of the Type III Transforming Growth Factor &#x3b2; Receptor (TGFRPIII) and uses thereof for producing red blood cells in vitro, genotoxicity analysis of chemicals, drug sensitivity assessment, and drug development. Also described herein are methods for producing the population of early-stage BFU-E cells and methods for producing red blood cells.

Efficient generation of human red blood cells via enriching peripheral blood erythroid progenitors

In vitro production of red blood cells with sortaggable proteins

Growing community of inventors

Cambridge, MA, United States of America

Hsiang-Ying Lee