University of California

California Institute of Technology

Siemens Medical Solutions Usa, Inc.

Riken Corporation

Cedars-sinai Medical Center

Fluidigm Corporation

National Institutes of Health (nih)

Yonsei University

Hsian-Rong Tseng

Hao Wang

Shutao Wang

Shuang Hou

Kan Liu

Min Song

Libo Zhao

Stephen R Quake

James R Heath

Hartmuth C Kolb

Paul S Weiss

R Michael Van Dam

Arkadij M Elizarov

Antoine Daridon

Leland W K Chung

Jiang Huang

Caius G Radu

Steven J Jonas

Johannes Czernin

Ker-Chau Li

Paul Wyatt

Michael E Phelps

Thomas G Graeber

Jinwoo Cheon

Natcha Wattanatorn

Xiaobin Xu

Qing Yang

Vatche Agopian

Arion F Hadjioannou (Chatziioannou)

Cong Fang

Yanju Wang

Nam T Vu

Kwang-Fu Clifton Shen

Chung-cheng Lee

Guodong Sui

Helen Su

Kuan-Ju Chen

Haichao Zhao

Shyh-Chyang Luo

Hsiao-Hua Yu

Tom Lee

Mitch A Garcia

Jake Lichterman

Yi-Tsung Lu

Zunfu Ke

Hao Ho

Jiefu Chen

Edwin M Posadas

Jiaoti Huang

Seung-hyun Noh

A method of transporting biomolecular cargo intracellularly into cells includes the operations of providing magnetic nanostructures (e.g., nanospears, nanostars, nanorods, and other nanometer-sized structures) carrying the biomolecular cargo thereon and applying an external magnetic field to move the magnetic nanostructures into physical contact with at least some of the cells (or the cells into the magnetic nanostructures). The magnetic nanostructures move into physical contact with a single cell, a subset of cells, or all cells. The external magnetic field may be applied by a moving permanent magnet although an electromagnetic may also be used. The biomolecular cargo may include a molecule, a plurality of molecules, or higher order biological constructs. For example, the biological construct may include DNA plasmids, small interfering RNA, proteins, or targeted nuclease gene-editing cargo such as zinc-finger nucleases, transcription activator-like effector nucleases, Cas9 protein, Cas9 mRNA, and associated guide RNA sequences.

Guided magnetic nanostructures for targeted and high-throughput intracellular delivery

Methods and kits for detecting hepatocellular carcinoma recurrence or metastasis, and of measuring markers of hepatocellular carcinoma, including markers of hepatocellular carcinoma recurrence or metastasis, in a blood sample obtained from a subject by (a) isolating circulating tumor cells (CTCs) by contacting a blood sample obtained from the subject with a set of capture antibodies, wherein the capture antibodies specifically bind asialoglycoprotein receptor (ASGPR), Glypican-3, and epithelial cell adhesion molecule (EpCAM); (b) contacting the isolated CTCs with an antibody that specifically binds vimentin; and (c) measuring the number of vimentin-positive CTC.

Phenotypic profiling of hepatocellular carcinoma circulating tumor cells for treatment selection

A method, system and computer-readable medium for assessing a disease condition of a cancer of a subject, including: receiving a blood sample from the subject; isolating a plurality of circulating tumor cells (CTCs) from the blood sample; measuring at least one of cell or cell nucleus sizes of each of the plurality of CTCs; determining a measured CTC size distribution of the plurality of CTCs based on the measuring; comparing the measured CTC size distribution to a reference CTC size distribution using a computer; and assigning the disease condition of the cancer of the subject based on the comparing.

Method of assessing disease condition of cancer

A system for isolating preselected cell types from a fluid sample that includes a plurality of cell types includes a cell-capture fluidic chip, and a chip holder configured to receive the cell-capture fluidic chip and to maintain the cell-capture fluidic chip with a substantially fluid-tight seal while in operation. The chip holder is further configured to release the cell-capture fluidic chip to be removed from the chip holder for further processing. The cell-capture fluidic chip includes a substrate, a laser micro-dissection membrane disposed on the substrate, and a channel-defining layer disposed on the laser micro-dissection membrane. The laser micro-dissection membrane has a surface adapted to capture preselected cell types preferentially over other cell types of the plurality of cell types. The channel-defining layer is removable from the laser micro-dissection membrane for further processing of the cell-capture fluidic chip.

Systems, methods and components for isolating cells from a fluid sample

A device for capturing preselected cell types from a fluid sample that includes a plurality of cell types includes a substrate, a plurality of nanowires at least one of attached to or integral with a surface of the substrate such that each nanowire of the plurality of nanowires has an unattached end, and a layer of temperature-responsive material formed on at least the unattached end of each of the plurality of nanowires. The layer of temperature-responsive material has a compact configuration at a first temperature and an expanded configuration at a second temperature so as to facilitate release of cells captured at the first temperature to be released at the second temperature.

Selective capture and stimulated release of circulating tumor cells on nanostructured devices

This invention relates, e.g., to a molecular delivery system comprising A. a substrate having a nanostructured surface region which comprises a plurality of nanostructures and, covalently attached to the substrate, multiple copies of a first member of a binding pair; and B. at least one vector nanoparticle which comprises, encapsulated therein, a molecule of interest, and on its surface, multiple copies of second member of the binding pair. Methods of using the molecular delivery system to deliver a molecule of interest to a cell are also described.

Catalytic delivery nanosubstrates (CDNS) for highly efficient delivery of biomolecules

A supramolecular approach has been developed for preparation of size-controllable nanoparticles, from three different molecular building blocks.

Supramolecular approach for preparation of size controllable nanoparticles

The present invention provides devices and methods for capturing rare cells. The devices and methods described herein can be used to facilitate the diagnosis and monitoring of metastatic cancers.

Device for capturing circulating cells

An immunocapture-based in vitro kinase assay on an integrated polydimethylsiloxane (PDMS) microfluidics platform that can reproducibly measure kinase activity from as few as 3,000 cells is described. For this platform, the standard radiometricP-ATP labeled phosphate transfer assay was adopted. Implementation on a microfluidic device required the development of methods for repeated trapping and mixing of solid-phase affinity micro beads. A solid state beta-particle camera imbedded directly below the microfluidic device was used to provide real-time quantitative detection of the signal from this and other microfluidic radio bioassays. The integrated device can measure ABL protein kinase activity from BCR-ABL positive leukemia patient samples, and can measure the small molecule phosphorylation such as phosphorylation of the deoxycytidine analogF-FAC by deoxycytidine kinase (dCK) isolated from cell lysates.

Integrated microfluidic radioassay and imaging platform for small sample analysis

The invention may provide a microfluidic mixer having a droplet generator and a droplet mixer in selective fluid connection with the droplet generator. The droplet generator comprises first and second fluid chambers that are structured to be filled with respective first and second fluids that can each be held in isolation for a selectable period of time. The first and second fluid chambers are further structured to be reconfigured into a single combined chamber to allow the first and second fluids in the first and second fluid chambers to come into fluid contact with each other in the combined chamber for a selectable period of time prior to being brought into the droplet mixer.

Accurate and rapid micromixer for integrated microfluidic devices

New microfluidic devices, useful for carrying out chemical reactions, are provided. The devices are adapted for on-chip solvent exchange, chemical processes requiring multiple chemical reactions, and rapid concentration of reagents.

Microfluidic chemical reaction circuits

Growing community of inventors

Los Angeles, CA, United States of America

Hsian-Rong Tseng