3m Innovative Properties Company

Hsi-Chou Liu

Stephen B Roscoe

Myungchan Kang

Evan D Brutinel

Jesse D Miller

Minghua Dai

Renee K Cline

Naiyong Jing

Joshua Erickson

Andrew W Vail

Tonya D Bonilla

Kelvin J Witcher

Francois Ahimou

A method is provided. The method includes providing an article, the article including a nonwoven substrate having a copolymer grafted thereto, the copolymer including interpolymerized monomer units of a quaternary ammonium-containing ligand monomers; an amide monomer; an oxy monomer; and a coating on the nonwoven substrate, the coating including a plurality of test microorganisms, an enzyme or a second substrate; and contacting the article with a detection medium for a period of time.

Methods to detect microorganisms

Assay devices are provided including a receptacle having a sample entry port; a plunger disposed within the receptacle; at least one reagent; a membrane attached to a first surface of the plunger; a wick providing capillary force; and a detection zone. The membrane includes a target conjugate zone in which affinity components are disposed. Capture compounds are immobilized in a detection zone, which is located between the target conjugate zone and the wick. A method of detecting a target analyte is also provided, including providing the assay device; providing a sample suspected to contain a target analyte; adding the sample onto the device; allowing the sample to travel along the membrane until the sample reaches the detection zone; immobilizing the target analyte through reaction of the target analyte with the capture compounds; reacting the immobilized target analyte with a reagent to generate a detectable signal; and detecting the generated signal.

Assay devices and method of detecting a target analyte

Growing community of inventors

Woodbury, MN, United States of America

Hsi-Chou Liu