The General Hospital Corporation

University of California

Harvard College

Other

Rijksuniversiteit

Howard M Goodman

James Haseloff

William J Rutter

John Shine

Andrea Brand

Norbert Perrimon

Peter H Seeburg

Raymond Pictet

Axel Ullrich

Frederick M Ausubel

Robert A Hallewell

Shiladitya DasSarma

Jeffrey C Edman

John Chirgwin

Hui-Hwa Chiang

Edmund Tischer

Theresa K Peterman

Hong Zhang

Pablo D Valenzuela

John D Baxter

Marc Charles Van Montagu

Laurence G Rahme

Lin Sun

Man-Wah Tan

Gunter Donn

Shalina Mahajan-Miklos

Long V Nguyen

Eliana Drenkard

Dominique Van Der Straeten

Tim Lynch

Tai-Ping Sun

Peter Horst

James L Roberts

Graeme Bell

Yun Lin

Richard Selden

Hui Cao

John Tsongalis

Ruth R Finkelstein

Ming-Li Wang

Disclosed is asse1 gene, which is responsible for protein and oil body biogenesis. The invention further provides methods for using the disclosed gene for modifying the components of plant storage reserve materials.

Nucleic acid encoding the arabidopsis SSE1 protein and a method of using it to modify plant storage reserves

Disclosed are bacterial virulence polypeptides and nucleic acid sequences (e.g., DNA) encoding such polypeptides, and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing such polypeptides to screen for antibacterial or bacteriostatic compounds.

Virulence-associated nucleic acid sequences and uses thereof

Identification, cloning and sequencing of the Arabidopsis ABI4 gene involved in seed response to abscisic acid (ABA) that regulates production of seed nutrient reserves and desiccation protectants. A method for regulating seed development, viability, stress-tolerance and nutrient reserves.

Transcription factor and method for regulation of seed development, quality and stress-tolerance

The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Inactive pro-ribozyme forms are also described.

Cell ablation using trans-splicing ribozymes

A process for producing a transgenic mouse which contains and expresses the human insulin hormone gene. The insulin gene is expressed only in the pancreas of the transgenic mouse and is regulated by glucose, glucagon, or other insulin affectors in a manner which is indistinguishable from that of normal mice. Progeny of the trangenic mice inherit the human insulin gene in a Mendelian manner, with approximately 50% of the mice of each litter expressing the human insulin gene. The weights of the transgenic mice, growth rate, feeding behavior, reproductive capability, and longevity appear indistinguishable from normal mice. The mice are useful for studies of pharmacokinetics of insulin expression and for investigations of possible drug interactions with glucose homeostasis.

Transgenic mice expressing human insulin

The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. Inactive pro-ribozyme forms are also described.

Trans-splicing ribozymes

The invention relates to the DNA and protein encoded by the GA4 locus. This protein is believed to be a member of the family of enzymes involved in the biosynthesis of the gibberellin family (GA) of plant growth hormones which promote various growth and developmental processes in higher plants, such as seed germination, stem elongation, flowering and fruiting. More specifically, the protein encoded by the GA4 locus is an hydroxylase. The invention also relates to vectors containing the DNA and the expression of the protein encoded by the DNA of the invention in a host cell. Additional aspects of the invention are drawn to host cells transformed with the DNA or antisense sequence of the invention, the use of such host cells for the maintenance, or expression or inhibition of expression of the DNA of the invention and to transgenic plants containing DNA of the invention. Finally, the invention also relates to the use of the protein encoded by the GA4 locus to alter aspects of plant growth.

GA4 DNA, protein and methods of use

GA4 DNA, protein and method of use

Disclosed is substantially pure DNA encoding a crucifer ACC synthase polypeptide; a promoter functional in immature plant tissues which is capable of ethylene induction; and methods of using such promoters to express recombinant proteins or RNA and to regulate ethylene-inducible events of a plant, e.g., fruit ripening or senescence, especially during early stages of plant development.

Crucifer ACC synthase and uses thereof

The present invention concerns the cloning and sequencing of DNA corresponding to the GA1 locus of A. thaliana which encodes ent-kaurene synthetase, to vectors containing said DNA, to vectors capable of expressing said DNA, and to hosts transformed with said vectors. The invention further concerns the use of the GA1 gene, and regulatory regions thereof, in the generation of chimeric and transgenic plants.

Recombinant gibberellin DNA and uses thereof

Growing community of inventors

Newton, MA, United States of America

Howard M Goodman