Bayer Corporation

Bayer Healthcare LLC

Howard A Cooper

Amy H Chu

Mary Ellen Warchal-Windham

Christina Blaschke

Mark S Vreeke

Barbara J Mikel

Wei-Sen Chu

Reagents for detecting an analyte are described. A reagent comprises (a) an enzyme selected from the group consisting of a flavoprotein, a quinoprotein, and a combination thereof; and (b) a mediator selected from the group consisting of a phenothiazine, a phenoxazine, and a combination thereof. In addition, reagents having good stability to radiation sterilization are described. Electrochemical sensors and sampling devices comprising such reagents, methods of producing a sterilized device including such reagents, and methods for detecting an analyte which utilize such reagents are described as well.

Reagents and methods for detecting analytes, and devices comprising reagents for detecting analytes

Disclosed is an improvement to the method for the detection of creatinine in which creatinine, in aqueous solution, is contacted with a dry reagent system of an indicator for creatinine. The assay is carried out at a pH above about 11.5 which is maintained by an alkaline material. The improvement involves packaging the reagent system with a material capable of absorbing CO.sub.2 and at least some ambient water vapor. This inhibits the formation of carbonic acid thereby reducing the neutralization of the alkaline reagent system during storage to increase the shelf life and decrease the variability of the system.

Use of CO.sub.2 absorbant for stabilization of dried alkaline reagent in

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Elkhart, IN, United States of America

Howard A Cooper