California Institute of Technology

Fluidigm Corporation

Pacific Biosciences of California, Inc.

Deepcell, Inc.

National Institutes of Health, a Component of the US Dept. of Health & Human Services

Hou-Pu Chou

Marc Alexander Unger

Stephen R Quake

Axel Scherer

Todd A Thorsen

Carl L Hansen

Mark L Adams

Markus M Enzelberger

David Fernandes

Takashi Whitney Orimoto

Annette Claire Grot

Mathieu Foquet

Ravi Saxena

Antoine Daridon

Michael Tzu Ru

Martin Pieprzyk

Jake Kimball

Robert Grossman

Mahyar Salek

Mahdokht Masaeli

Phillip Lam

Soroush Kahkeshani

Hany Ramez Nassef

Ian D Manger

Geoffrey Richard Facer

Brian Fowler

Jing Wang

Geoffrey Facer

Jiang Huang

Jian Liu

Kevin Farrell

Anne Y Fu

Yish-Hann Liau

William Throndset

Tim Woudenberg

Andrew Paul May

Michael Ybarra Lucero

Yong Yi

Prashast Khandelwal

Kathy Yokobata

Mark A Unger

Philip Lam

Dave Fernandes

Syed Tariq Shafaat

Arrays of integrated analytical devices and their methods for production are provided. The arrays are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The integrated devices allow the highly sensitive discrimination of optical signals using features such as spectra, amplitude, and time resolution, or combinations thereof. The arrays and methods of the invention make use of silicon chip fabrication and manufacturing techniques developed for the electronics industry and highly suited for miniaturization and high throughput.

Arrays of integrated analytical devices and methods for production

The present disclosure provides systems and methods for sorting a cell. The system may comprise a flow channel configured to transport a cell through the channel. The system may comprise an imaging device configured to capture an image of the cell from a plurality of different angles as the cell is transported through the flow channel. The system may comprise a processor configured to analyze the image using a deep learning algorithm to enable sorting of the cell.

Systems and methods for particle analysis

The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.

Apparatus and methods for conducting assays and high throughput screening

A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

Microfabricated elastomeric valve and pump systems

Methods and systems are provided for conducting a reaction at a selected temperature or range of temperatures over time. An array device is provided. The array device contains separate reaction chambers and is formed as an elastomeric block from multiple layers. At least one layer has at least one recess that recess has at least one deflectable membrane integral to the layer with the recess. The array device has a thermal transfer device proximal to at least one of the reaction chambers. The thermal transfer device is formed to contact a thermal control source. Reagents for carrying out a desired reaction are introduced into the array device. The array device is contacted with a thermal control device such that the thermal control device is in thermal communication with the thermal control source so that a temperature of the reaction in at least one of the reaction chamber is changed as a result of a change in temperature of the thermal control source.

Integrated chip carriers with thermocycler interfaces and methods of using the same

A microfabricated fluidic unidirectional valve includes a microfabricated elastomer material having a flow through channel. The microfabricated fluidic unidirectional valve also includes an elastomer flap attached to the elastomer material in the flow through channel. The elastomer flap forms a seal in the flow through channel to prevent fluid from flowing in a first direction through the flow through channel and to allow fluid flow in a second direction through the flow through channel.

Microfabricated fluidic circuit elements and applications

Microfluidic devices are described that include a rigid base layer, and an elastomeric layer on the base layer. The elastomeric layer may include at least part of a fluid channel for transporting a liquid reagent, and a vent channel that accepts gas diffusing through the elastomeric layer from the flow channel and vents it out of the elastomeric layer. The devices may also include a mixing chamber fluidly connected to the fluid channel, and a control channel overlapping with a deflectable membrane that defines a portion of the flow channel, where the control channel may be operable to change a rate at which the liquid reagent flows through the fluid channel. The devices may further include a rigid plastic layer on the elastomeric layer.

Microfluidic mixing and reaction systems for high efficiency screening

Growing community of inventors

Sunnyvale, CA, United States of America

Hou-Pu Chou