Degussa Aktiengesellschaft

Gesellschaft Fur Biotechnologische Forschung Mbh

Society for Biotechnical Research

Degussa Aktiengelleschaft

Gesellschaft Fur Biotechnologisch Forschung

Degussa Aktiengesellschaft and Gesellschaft Fur Biotechnologische

Horst Schutte

Maria-Regina Kula

Werner Hummel

Wolfgang Leuchtenberger

Hubert Mayer

The invention is directed to obtaining phenylalanine-dehydrogenase containing microorganisms by a special selection process comprising obtaining microorganisms, especially strains of Rhodococcus and the phenylalanine-dehydrogenase obtained in them. With the help of this enzyme there are obtained L-.alpha.-aminoacids from the corresponding ketocarboxylic acids by reductive amination.

Rhodococcus microorganisms that produce phenylalanine dehydroganase

A microbiologically produced .alpha.-acetylamino cinnamic acid acylase and a method for its production from Brevibacterium species NCIB 12246 or NCIB 12247. The new enzyme can be used as constituent of a coupled enzyme system for enzymatic conversions which run via the intermediary stage .alpha.-imino-.beta.-phenylpropionic acid or phenylpyruvic acid.

Microbiologically produced .alpha.-acetylamino cinnamic acid acylase,

The subject matter of the invention is a new D(-)-mandelate-dehydrogenase and obtaining it from Lactobacillus curvatus DSM 20019 (obtainable from the German Collection of Microorganisms). The new enzyme can be used for the enzymatic conversion of D(-)-mandelic acid and various other D-2-hydroxycarboxylic acids into the corresponding 2-ketocarboxylic acids or from benzoyl formate and various other 2-ketocarboxylic acids conversion to the corresponding D-2-hydroxycarboxylic acids.

Microbiologically produced d(-)-mandelate-dehydrogenase process for

The enzyme D-2-hydroxy-4-methylpentanoic acid dehydrogenase has been prepared by culturing readily available Lactobacillus or Leuconostoc, microorganisms, such as Lactobacillus casei ssp. pseudoplantarum and Leuconostoc mesenteroides. The microbiologically produced enzyme has special characteristics and is capable of converting D-2-hydroxycarboxylic acids, such as D-2-hydroxy-4-methylpentanoic acid to the corresponding 2-ketocarboxylic acid and is capable of enzymatically converting 2-ketocarboxylic acids, such as 2-keto-4-methylpentanoic acid and 2-ketobutyric acid to the corresponding D-2-hydroxycarboxylic acid.

Microbiologically produced D-2-hydroxy-4-methylpentanoic acid

The invention is directed to a microbiologically produced L-phenylalanine-dehydrogenase and a process for its recovery from Brevibacterium species DSM 2448. The new enzyme can be used for the enzymatic conversion of phenyl pyruvic acid, p-hydroxyphenyl pyruvic aid, indolyl pyruvic acid or 2-keto-4-(methylmercapto)-butyric acid into the corresponding L-.alpha.-aminocarboxylic acids.

Microbiologically produced L-phenylalanine-dehydrogenase, process for

The purpose of the invention is the new enzyme L-2-hydroxy-4-methylpentanoic acid-dehydrogenase and its recovery from Lactobacillus confuses. The new enzyme can be used to enzymatically change L-2-hydroxy-4-methylpentanoic acid and various other L-2-hydroxy-carboxylic acids into the corresponding 2-ketocarboxylic acids or 2-keto-4-methylpentanoic acid and various other 2-ketocarboxylic acids into the corresponding L-2-hydroxycarboxylic acids.

L-2-hydroxy-4-methylpentanoic acid-dehydrogenase, process for obtaining

ECoRI restriction endonuclease is produced with certain mutant strains of E.coli which are not antibiotic resistant and do produce the said enzyme which is obtained from the E.coli mutant cells without destroying the cells by extraction procedures.

Growing community of inventors

Salzgitter, Germany

Horst Schutte