Bayer Healthcare LLC

Ascensia Diabetes Care Holdings Ag

Hope Spradlin

Huan-Ping Wu

Christine D Nelson

Eric Anthony Maurer

Amy H Chu

Andrew J Edelbrock

The present invention relates to electrochemical sensor strips and methods of determining the concentration of an analyte in a sample or improving the performance of a concentration determination. The electrochemical sensor strips may include at most 8 &#x3bc;g/mmof a mediator. The strips, the strip reagent layer, or the methods may provide for the determination of a concentration value having at least one of a stability bias of less than &#xb1;10% after storage at 50&#xb0; C. for 4 weeks when compared to a comparison strip stored at &#x2212;20&#xb0; C. for 4 weeks, a hematocrit bias of less than &#xb1;10% for whole blood samples including from 20 to 60% hematocrit, and an intercept to slope ratio of at most 20 mg/dL. A method of increasing the performance of a quantitative analyte determination also is provided.

Electrochemical test sensor

The present invention relates to methods of determining the concentration of an analyte in a sample or improving the performance of a concentration determination. The electrochemical sensor strips may include at most 8 &#x3bc;g/mmof a mediator. The strips, the strip reagent layer, or the methods may provide for the determination of a concentration value having at least one of a stability bias of less than &#xb1;10% after storage at 50&#xb0; C. for 4 weeks when compared to a comparison strip stored at &#x2212;20&#xb0; C. for 4 weeks, a hematocrit bias of less than &#xb1;10% for whole blood samples including from 20 to 60% hematocrit, and an intercept to slope ratio of at most 20 mg/dL.

Methods of determining analyte concentration having enhanced stability and hematocrit performance

A test sensor reagent for measuring the concentration of analytes in body fluids includes cellulose polymers for improving the stability of the test sensor and reducing the total assay time. The test sensor reagent also includes an enzyme, an electron transfer mediator and a rheological additive.

Test sensor reagent having cellulose polymers

A reagent composition containing GDH-PQQ as an enzyme-co-factor and screen-printed on working and counter electrodes of electrochemical biosensors, maintains activity of the enzyme reagents by proper selection of components. A preferred composition includes hydrophilic polymers, amorphous untreated silica, buffers, surfactants, and a mediator. For example, the biosensor is useful in the amperometric determination of glucose.

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Granger, IN, United States of America

Hope Spradlin