Canon U.S. Life Sciences, Inc.

Canon U.s.a., Inc.

Hongye Liang

Kenton C Hasson

Johnathan Stuart Coursey

Eric D Schneider

Bradley Scott Thomas

Shulin Zeng

Gregory H Owen

Scott Corey

Sami Kanderian

Conrad Laskowski

Brian Bean

Brian P Murphy

Hiroshi Inoue

Ben Lane

Alex K Flamm

Ivor T Knight

Weidong Cao

Takayoshi Hanagata

Ying-Xin Wang

Franklin Regan

Kunihiro Sakai

George Maltezos

Arvind Virmani

Hanyoup Kim

Harini Shandilya

Melissa Gosse

Jeremy Savage

Makoto Ogusu

Yoichi Murakami

Jason Zsak

Abhijit Dohale

Brian Scrivens

Christopher Sneeder

Denis Alias

Yasuyuki Numajiri

The present invention relates to sample-to-answer systems, devices, cartridges, and method of using the same for detecting the presence of microorganisms in a sample, such as bacteria.

Sample-to-answer system for microorganism detection featuring target enrichment, amplification and detection

The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.

System and method for serial processing of multiple nucleic acid assays

This invention relates to systems and methods for imaging sample materials within a microfluidic device during an assay reaction process. In accordance with certain aspects of the invention, images are formed with a pixel array and a region of interest ('ROI') is defined within the pixel array. Image values, such as fluorescent intensity, can be computed as averages of individual pixel values within the ROI. Where the ROI is subject to non-uniform conditions, such as non-uniform heating, the ROI can be divided into sub-ROIs which are sufficiently small that the condition is uniform within the sub-ROI.

Optical system for high resolution thermal melt detection

The present invention, in one aspect, provides methods and systems for controlling slugs using temperature dependent fluorescent dyes. In some embodiments, the present invention uses one or more techniques to enhance the visibility of slugs, enhance a system's ability to differentiate between slugs, and enhance a system's ability to identify the positions of slugs.

Slug control during thermal cycling

A microfluidic chip includes microfluidic channels, elements for thermally and optically isolating the microfluidic channels, and elements for enhancing the detection of optical signal emitted from the microfluidic channels. The thermal and optical isolation elements may comprise barrier channels interposed between adjacently-arranged pairs of microfluidic channels for preventing thermal and optical cross-talk between the adjacent microfluidic channels. The isolation element may alternatively comprise reflective film embedded in the microfluidic chip between the adjacent microfluidic channels. The signal enhancement elements comprise structures disposed adjacent to the microfluidic channels that reflect light passing through or emitted from the microfluidic channel in a direction toward a detector. The structures may comprise channels or a faceted surface that redirects the light by total internal reflection or reflective film material embedded in the microfluidic chip.

Microfluidic chip features for optical and thermal isolation

Chip station plate with LED assembly

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Clarksville, MD, United States of America

Hongye Liang