E.i. Dupont De Nemours and Company

Danisco US Inc.

Butamax Advanced Biofuels LLC

Nutrition & Biosciences USA 4, Inc.

Dupont Industrial Biosciences Usa, LLC

Hongxian He

Mark Scott Payne

Yefim Brun

Thomas Scholz

Quinn Qun Zhu

Yougen Li

Steven Cary Rothman

Ryan L Frisch

Jessica McElvain

Howard Glenn Damude

Lori Ann Maggio-Hall

Mark J Nelson

Larry Cameron Anthony

Arthur Leo Kruckeberg

Der-Ing Liao

Ranjan Patnaik

Lixuan Lisa Huang

Daniel P Okeefe

Hong Peng Wang

Qiong Cheng

Stephen R Fahnestock

Chris Leeflang

Marc Anton Bernhard Kolkman

Kristy N Kostichka

Frank Wouter Koopman

Steven D Doig

Michael W Bostick

Narendra S Yadav

Zhixiong Xue

Daniel P O'Keefe

Hongxiang Zhang

Seung-Pyo Hong

Brian James Paul

Dana M Walters Pollak

Pamela L Sharpe

Lisa L Huang

Melissa D Bosak

Qun Zhu

Yehong Jamie Wang

Jessica Mcelvain

Katharine J Gibson

Daniel P O'keefe

Compositions and methods are provided for variant Cas systems and elements comprising such systems, including, but not limiting to, Cas endonuclease variants, guide polynucleotide/Cas endonuclease complexes comprising Cas endonuclease variants, as well as guide polynucleotides and guide RNA elements that can interact with Cas endonuclease variants. Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system comprising a Cas9 endonuclease variant to provide an effective system for modifying or altering target sequences within the genome of a cell or organism.

Cas9 variants and methods of use

The present disclosure is generally related to compositions and methods for obtainingcells/strains having increased protein production capabilities. Certain embodiments of the disclosure are related to genetically modifiedcells/strains derived from parentalcells/strains comprising a variant rghR2 gene.

Compositions and methods for increased protein production in

Compositions and methods for increased protein production in bacillus licheniformis

Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.

Glucosyltransferase enzymes for production of glucan polymers

Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.

Host cells and methods for production of isobutanol

Short single chain peptides having affinity for a target surface often lack the binding durability required for certain commercial applications. One way to improve durability is to promote multivalent binding by linking together binding sequences using peptide linkers. However, the resulting single chain binding peptides often suffer from linker entropy. It has been discovered that the use of rigid peptide linkers when linking together multiple binding sequences enhances the binding affinity of the resulting single chain peptide.

Peptide linkers for effective multivalent peptide binding

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Wilmington, DE, United States of America

Hongxian He