Chinese Academy of Sciences

Hongtao Xu

Xueli Zhang

Yanhe Ma

Xinna Zhu

Jinlei Tang

Tao Sun

Jing Zhao

Qingyan Li

Zaigao Tan

Guanping Dai

Pingping Liu

The invention relates to the field of producing succinate byfermentation. Specifically, the invention provides an engineered recombinantfor producing succinate, wherein saidcontains one or more of the following modifications: a) enhanced activity of the protein(s) encoded by the gene(s) involved in pentose phosphate pathway (PPP), b) enhanced activity of the protein encoded by sthA gene, and optionally c) mutant lpdA gene. The invention also relates to use of the engineered recombinantfor producing succinate, and a method of using the engineered recombinantfor producing succinate.

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Provided is recombinantcomprising a mutated ldhA gene. Disclosed are use of the recombinantin the preparation of D-lactate, and method of preparing D-lactate by employing the recombinant. In the method of preparing D-lactate, fermentation can be conducted at a high temperature (42-50&#xb0; C.), and aqueous ammonia or sodium hydroxide can be used as a neutralizer during the fermentation.

Provided are a recombinant strain for preparing a terpenoid, and method for constructing the recombinant strain. Also provided is a recombinant bacterium 1, the recombinant bacterium 1 being a recombinant bacterium obtained in order to improve the enzymatic activity of &#x3b1;-ketoglutarate dehydrogenase inor the mutant thereof. The method for improving the enzymatic activity of &#x3b1;-ketoglutarate dehydrogenase inor the mutant thereof is replacing the original regulating element of the ketoglutarate dehydrogenase gene (sucAB) inor the mutant thereof with any of the following regulating elements: artificial regulating element M1-46, M1-37, and M1-93. Also provided are a plurality of recombinant bacteria. By improving the enzymatic activity of &#x3b1;-ketoglutarate dehydrogenase, succinic acid dehydrogenase and transaldolase therein and improving the ability of a cell to synthesize NADPH and ATP, the efficiency of the MEP pathway and the production capacity of terpenoid are improved.

Recombinant microorganism for preparing terpenoid and method for constructing recombinant microorganism

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Tianjin, China

Hongtao Xu