Shandong University

Shandong Institute for Food and Drug Control

Avanc Pharmaceutical Co., Ltd

Hongming Du

Feng Shi

Lianli Chi

Baojian Hang

Weijian Wang

Ruiqing Xian

Liping Gong

Congcong Wang

Fengshan Wang

Pengfei You

Qunye Zhang

The present invention provides a snake venom thrombin marker peptide ofand an application of the snake venom thrombin-like enzyme in identifying species of Hemocoagulase for Injection. The application includes the following steps of: dissolving a to-be-detected sample and a reference substance of the marker peptide respectively to prepare a test solution and a reference solution, and conducting alkylation reduction on the test solution and the reference solution with dithiothreitol and iodoacetamide; after diluting products with an ammonium bicarbonate solution, adding enzyme for hydrolysis; and after enzymolysis is finished, conducting centrifugation at a high speed, and injecting a supernatan into a liquid chromatography-mass spectrometer for analysis. This method is simple, convenient and rapid, is strong in specificity, fills the gap in identifying the source of species of the snake venom thrombin-like enzyme ofand improves the quality control level.

Snake venom thrombin-like enzyme marker peptide ofand its application in the species identification of hemocoagulase for injection

The present invention relates to the technical field of chemical analysis and quantitative detection, in particular to a quantitative detection method for snake venom thrombin-like enzyme (SVTLE) from. The quantitative detection method for the SVTLE includes the following steps of taking a reference substance of marker peptide for the SVTLE fromwith an amino acid sequence of LDSPVSNSAHIAPLSLPSSAPSVGSVCR, and preparing a series of reference solutions with different concentrations; adding the reference solutions in test solutions respectively for enzymolysis, and then taking a supernatant after enzymolysis as a series of solutions to be detected; and adding the solutions to be detected in a liquid chromatogram-mass spectrometer, and then selecting a qualitative ion pair and a quantitative ion pair to detect contents of marker peptide in the solutions to be detected.

Quantitative detection method for snake venom thrombin-like enzyme (SVTLE)

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Liaoning, China

Hongming Du