Xintrum Pharmaceuticals, Ltd.

Hongliang Sun

Yi Zhang

Yuesheng Wang

Hai Chen

Wenchao Liu

A gene combination capable of high-efficiency expression of rhNGF is provided to optimize gene expression regulation components of a recombinant human nerve growth factor. The gene combination enhances expression of rhNGF. As shown by experiments, the gene combination is capable of high-efficiency expression of a recombinant human nerve growth factor (rhNGF) with natural activity in a eukaryotic expression system.

Gene combination capable of high-efficiency expression of recombinant human nerve growth factor

A method for removing precursors in recombinant human nerve growth factor (rhNGF) by hydrophobic interaction chromatography (HIC) is provided, where a Chinese hamster ovary (CHO) cell culture is processed by column chromatography for preliminary purification, and the pretreated sample obtained therefrom is further processed in a HIC column by washing the sample and then eluting the HIC column.

Growing community of inventors

Nanjing, China

Hongliang Sun