University of Shanghai for Science and Technology

Hongli Yan

Weijie Li

Jing Shen

Qin Qi

Zhixin Li

Yanbin Qin

Baolin Liu

A method, system and device for programmed cell freezing is disclosed. The method includes: S1, acquiring a cooling rate k and a temperature distribution function T=a×h+b×h+c of a heat preservation chamber containing liquid nitrogen; S2, controlling a cell preservation tube to lower in the chamber at a speed of v=(k−b)/2ah, and acquiring temperatures Tand Tinside and outside the tube at a same time and at a same height in real time during the lowering of the tube; S3, determining whether a difference Δt between Tand Texceeds a preset temperature difference threshold, if yes, proceeding to S4, and if no, proceeding to S2 until the tube reaches a position corresponding to a preset temperature T; and S4, controlling the cell preservation tube to stop until the difference Δt between Tand Tis less than or equal to the temperature difference threshold, and continuing S2.

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Shanghai, China

Hongli Yan