Harvard College

Massachusetts Institute of Technology

The Broad Institute, Inc.

The Brigham and Women's Hospital, Inc.

The Charles Stark Draper Laboratory, Inc.

Other

Samsung Electronics Co., Ltd.

University of California

Element Six Gmbh

Element Six Technologies Limited

Hongkun Park

Charles M Lieber

Yi Cui

Donhee Ham

Qingqiao Wei

Jeffrey T Abbott

Wenjie Liang

Aviv Regev

Mikhail D Lukin

Alexander K Shalek

Wenxuan Wu

Tianyang Ye

Vijay K Kuchroo

Robert C Devlin

Philip Kim

Jellert Gaublomme

Han Sae Jung

Nir Yosef

Alexander Arthur High

Federico Capasso

Chao Wang

Mohammadreza Khorasaninejad

Soonwon Choi

Steven Andrew McCarroll

Anindita Basu

Trond Andersen

Christopher B Ford

Evan Macosko

Youjin Lee

Igor Lovchinsky

Marsela Jorgolli

Jinseong Heo

David A Weitz

Seongjun Park

Xiangfeng Duan

Minhyun Lee

Marko Loncar

Yu Huang

Matthew Lee Markham

Ronald Lee Walsworth

Ana Carrizosa Anderson

Peter Christian Maurer

Jinseok Lee

Ruffin E Evans

Amir Yacoby

Alexander S Zibrov

Henry Julian Hinton

Myung-Han Yoon

David Patterson

Georg Kucsko

Patrick Maletinsky

Alastair Douglas Stacey

Nathalie De Leon

Rahul Satija

Bo Loren Dwyer

Vladimir Manucharyan

Brendan John Shields

Javier Sanchez

Jeffrey J Urban

Qian Gu

Wan Soo Yun

Wenji Liang

Mark Gudiksen

Lincoln J Lauhon

Jianfang Wang

Nicole C Joller

David C Smith

Deli Wang

Chuan Wu

Zhaohui Zhong

James Kaminski

Hyun Ji Noh

Minako Kubo

Ling Qin

Rivka Bekenstein

Arthur Safira

Giovanni Scuri

Dominik S Wild

Andrey Sushko

Susanne F Yelin

Keith Krenek

Shimon Jacob Kolkowitz

Ryan J Gelly

Quirin P Unterreithmeier

Alexander Sushkov

Nicholas Chisholm

Yiwen Chu

Birgit Judith Maria Hausmann

EunGyeong Yang

Jacob Robinson

Amy Sutton

Elana K Urbach

Alexander Kann Shalek

Steven Andrew Mccarroll

Evan Zane Macosko

Disclosed herein are an apparatus for electrically assessing and/or manipulating cells. One aspect is directed to electrically mapping cells on the surface of the semiconductor substrate via cross-electrode impedance measurements. Further according to some aspects, the electrode array allows for spatially addressable electrical stimulation and/or recording of electrical signals in real-time using the CMOS circuitry. Some of these aspects are directed to using an electrode array to perform cell patterning through electrochemical gas generation, and extracellular electrochemical mapping.

Systems and methods for patterning and spatial electrochemical mapping of cells

Disclosed herein are semiconductor devices to provide a CMOS-compatible, wafer-scale, multi-well platform that can be used for biomedical or other applications, and methods to operate the same. In some embodiments, circuitry is provided underneath a multiple-well array to electrically interface with electrodes in the wells. To interface with electrodes in a large array, circuitry may be fabricated on a single silicon (Si) wafer having a dimension that is at least the same or larger than that of the multiple-well array. According to one aspect of the present disclosure, standard CMOS fabrication process such as those known to be used in a standard semiconductor foundry may be used without expensive customization for complex fabrication procedures. This may help the production cost to be lowered in some cases.

Complementary metal-oxide-semiconductor (CMOS) multi-well apparatus for electrical cell assessment

An optical device useful for spatial light modulation. The device comprises: a semiconductor layer having a first surface and a second surface, the semiconductor having an electric field-dependent resonance wavelength; a first electrode electrically connected to the semiconductor layer; a first insulating layer adjacent to the first surface of the semiconductor layer, and a second insulating layer adjacent to the second surface of the semiconducting layer, the first and the second insulating layers each being optically transparent at the resonance wavelength; a first group of at least one gate electrodes disposed adjacent to the first insulating layer, and a second group of at least one gate electrodes disposed adjacent to the second insulating layer, each gate electrode being at least 80% optically transparent at the resonance wavelength; wherein the first and the second groups of gate electrodes, taken together, form at least two regions in the semiconductor layer, an electrostatic field in each of the at least two regions being independently controllable by application of voltage to the first and the second groups of gate electrodes, the at least two regions abutting each other along at least one boundary.

Fast spatial light modulator based on atomically thin reflector

Methods and systems for monitoring the activity of electrogenic networks are described. One representative system includes an array of electrode coupled to an analyzer having a stimulator and a receiver. The electrode is placed in contact with an electrogenic cell. The electrodes can be shaped as nanowires, tubes, cavities and/or cones. The analyzer may be configured to operate in a voltage stimulation mode, in which the cells are stimulated via voltages and monitored via current, or in a current stimulation mode, in which the cells are stimulated via currents and monitored via voltages. The analyzers may be arranged as single-stage amplifiers, and may include a feedback loop shared between the stimulation signal path and the sensing signal path. The feedback loop may be arranged to provide overlapping stimulation and sensing of the electrogenic network's cells.

Electronic circuits for analyzing electrogenic cells and related methods

The present invention generally relates to nanowires. In one aspect, the present invention is generally directed to systems and methods of individually addressing nanowires on a surface, e.g., that are substantially upstanding or vertically-oriented with respect to the surface. In some cases, one or more nanowires may be individually addressed using various integrated circuit ('IC') technologies, such as CMOS. For example, the nanowires may form an array on top of an active CMOs integrated circuit.

Integrated circuits for neurotechnology and other applications

A method of fabricating a visible spectrum optical component includes: providing a substrate; forming a resist layer over a surface of the substrate; patterning the resist layer to form a patterned resist layer defining openings exposing portions of the surface of the substrate; performing deposition to form a dielectric film over the patterned resist layer and over the exposed portions of the surface of the substrate, wherein a top surface of the dielectric film is above a top surface of the patterned resist layer; removing a top portion of the dielectric film to expose the top surface of the patterned resist layer and top surfaces of dielectric units within the openings of the patterned resist layer; and removing the patterned resist layer to retain the dielectric units over the substrate.

Atomic layer deposition process for fabricating dielectric metasurfaces for wavelengths in the visible spectrum

Methods and systems for stimulating and monitoring electrogenic cells are described. Some systems for stimulating electrogenic cells are based on the injection of electric currents into the cells via electrodes connected to the cells. Such stimulators may comprise an impedance element having an input terminal and an output terminal coupled to an electrode, and a voltage follower coupled between the input terminal and the output terminal of the impedance element, the voltage follower being configured to maintain a substantially constant voltage between the input terminal and the output terminal of the impedance element. The impedance element may comprise one or more switched capacitors at least in some embodiments. In some embodiments, the voltage follower may be implemented using a source follower.

Current-based stimulators for electrogenic cells and related methods

Apparatuses for cell mapping via impedance measurements and methods to operate the same

The present invention generally relates to a combination of molecular barcoding and emulsion-based microfluidics to isolate, lyse, barcode, and prepare nucleic acids from individual cells in a high-throughput manner.

Droplet-based method and apparatus for composite single-cell nucleic acid analysis

This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

T cell balance gene expression, compositions of matters and methods of use thereof

A light-emitting device includes a substrate including a photonic cavity and configured to function as a gate, an active layer including a two-dimensional material, a first conductive contact, and a second conductive contact. The wavelength range of light generated by the light-emitting device may be narrowed based on the photonic cavity being included in the substrate, and the intensity and wavelength range of the generated light may be controlled based on the substrate functioning as a gate.

Light-emitting device comprising photonic cavity

Growing community of inventors

Lexington, MA, United States of America

Hongkun Park