Capitalbio Corporation

Tsinghua University

Sichuan University

Hongju Guo

Jing Cheng

Wanli Xing

Lei Wang

Jian Gao

Shuo Li

Yang Wang

Xiang Chen

Jiang Zhu

Liang Bai

Xinying Zhou

Xiaolei Zhao

Tengfei Guo

Baolian Li

Guohao Zhang

Zhengde Feng

Bin Zhuang

A liquid storage and controlled-release device and a biological detection chip. The liquid storage and controlled-release device comprises a liquid storage capsule with a liquid storage body which is deformable under a pressure, and a sealing layer for sealing the liquid storage body. A support platform is provided right below the liquid storage capsule and tightly connected with the liquid storage capsule, wherein, a directional release chamber is provided at the middle of the support platform, and the directional release chamber is provided with a guiding chamber for collecting the liquid and a sharp edge for inducing break. Compared with the conventional technology, the opening for liquid release is at the end far away from the rotation center, so that the liquid can be released completely by the centrifugal force, ensuring the quantitative release of stored liquid and reducing the influence on the accuracy of the subsequent detection.

Liquid storage and controlled-release device and biological detection chip

In one aspect, the present disclosure provides an integrated microfluidic device for nucleic acid amplification and microarray detection. In one aspect, the device comprises: (1) a microchip configured to process reagents, comprising a plurality of reservoirs, channels, valves, and/or fluid interfaces; (2) an amplification chamber for PCR, carried out in a detachable tube assembled on the microchip through a joint; and (3) a microarray chamber comprising a microarray and a reaction chamber. In some embodiments, these features are interconnected to allow transportation of reagents for nucleic acid amplification and hybridization detection functions in a closed system. In one aspect, the integrate device herein overcomes the problem of contamination during the amplification and hybridization reactions.

Integrated microfluidic device for target amplification and microarray detection

Growing community of inventors

Beijing, China

Hongju Guo