Nat Diagnostics, Inc.

Ionian Technologies, Inc.

Geneohm Sciences, Inc.

Andcare, Inc.

Other

Esa Biosciences, Inc.

Genohm Sciences

Biotronic Technologies, Inc.

Honghua Zhang

Andrew P Miller

Richard B Roth

Robert W Henkens

John P O'Daly

Donald M Crothers

Thomas N Stewart

R Michael Roe

Deborah M Thompson

Marek Wojciechowski

Chunnian Shi

Steven E Wegner

Najih Naser

R Erik Holmlin

Rebecca Sundseth

Daiwei Shen

Junguo Zhao

Jarrod Provins

Erik R Holmlin

The technology relates in part to methods and compositions for isothermal amplification of nucleic acids.

Isothermal amplification components and processes

This invention relates to methods and compositions for detecting the presence or absence of a Respiratory Syncytial Virus target nucleic acid in a biological sample using isothermal nucleic acid amplification.

Nicking and extension amplification reaction (NEAR) of respiratory syncytial virus species

This invention relates to compositions, methods and kits for detecting the presence or absence of a bacterial species in a biological sample using isothermal nucleic acid amplification.

Nicking and extension amplification reaction (near) ofspecies

A method includes combining a polynucleotide and an amplification reagent mixture to form a reaction mixture, wherein the reaction mixture comprises reversibly bound divalent ions in solution, and adjusting the pH of the reaction mixture to release the reversibly bound divalent ions, thereby initiating amplification of the polynucleotide.

Nucleic acid amplifications

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The present disclosure provides methods and compositions for conducting an assay to detect a polynucleotide. In particular, ruthenium complexes having reduction potentials that do not coincide with the reduction potential of molecular oxygen are disclosed and amperometric techniques for their use are described. In preferred embodiments, the ruthenium complex is ruthenium (III) pentaamine pyridine and the polynucleotide that is detected is DNA. Further, techniques for enhancing detectable contrast between hybridized and unhybridized nucleic acids are disclosed. In particular, the use of elongated target strands as well as the use of uncharged probe strands are discussed.

Electrochemical method to measure DNA attachment to an electrode surface in the presence of molecular oxygen

An electrochemical detection system which specifically detects selected nucleic acid segments is described. The system utilizes biological probes such as nucleic acid or peptide nucleic acid probes which are complementary to and specifically hybridize with selected nucleic acid segments in order to generate a measurable current when an amperometric potential is applied. The electrochemical signal can be quantified.

Electrochemical detection of nucleic acid sequences

The present disclosure provides methods and compositions for conducting an assay to detect nucleic acid hybridization in the presence of oxygen. In particular, ruthenium complexes having a reduction potential that does not coincide with the reduction potential of molecular oxygen are disclosed and amperometric techniques for their use are described. In preferred embodiments, the ruthenium complex is ruthenium (III) pentaamine pyridine and the nucleic acid hybridization event that is detected is DNA hybridization. Further, techniques for enhancing detectable contrast between hybridized and unhybridized nucleic acids are disclosed. In particular, the use of elongated target strands as well as the use of uncharged probe strands are discussed.

The present invention relates to devices comprising electrosensors containing capture reagents, their preparation thereof, and their use for detecting, preferably, quantitative measurement, of analyte in a liquid sample. In particular, the invention relates to an enzyme electrosensor, e.g., electroimmunosensor, device for electrochemical detection and preferably, real-time measurement, which is suitable for use at point-of-care settings by unskilled personnel.

Devices and methods for detecting analytes using electrosensor having capture reagent

Growing community of inventors

San Diego, CA, United States of America

Honghua Zhang