Joinstar Biomedical Technology Co., Ltd.

Shanghai Allrun Nano Science and Technology Co., Ltd.

Shanghai Mag-gene Nanotech Co., Ltd.

Hongchen Gu

Hong Xu

Longlan Cui

Ying Zhu

Hao Yang

Yefei Wang

Dingshengzi Zhang

Gaolian Xu

A nucleic acid amplification blocker for detecting a low-abundance mutation sequence and an application thereof in detecting a low-abundance mutation sequence are provided. The nucleic acid amplification blocker is an oligonucleotide modified by locked nucleic acid (LNA), and the matching region of the nucleic acid amplification blocker is located between amplified sequences. The nucleic acid amplification blocker is completely complementary to wild-type gene sequence, and contains at least one mismatch with mutant sequence. The nucleic acid amplification blocker has a great difference in affinity with mutant nucleic acid sequence/wild-type nucleic acid sequence, so as to achieve the purpose of highly selective amplification/enrichment of mutant sequence in samples. The nucleic acid amplification blocker has more significant detection effect on deletion mutation and insertion mutation.

Nucleic acid amplification blocker for detecting low-abundance mutation sequence and application thereof

Disclosed is a carrier particle, comprising a sphere and a sub-sphere on the surface of the sphere; the sphere and/or at least one sub-sphere comprise at least one type of quantum dots. Also disclosed is a method for preparing the carrier particle, mainly by connecting at least one nanosphere to a microsphere, and the microsphere and/or the nanosphere are loaded with at least one type of quantum dots.

Carrier particle and preparation method thereof

Polymerization process for preparing mono-disperse organic/inorganic nanocomposite microspheres in the field of nano technology is disclosed. The process comprises preparing of two different miniemulsion systems of A with inorganic nanoparticles and B with polymeric monomers in advance; adding water-soluble initiator into miniemulsion A, stirring till complete dissolution finally, heating the mixed system to a given temperature for isothermal reaction. The grain size of the as-prepared superparamagnetic FeO/polystyrene using to present invention is homogeneous and controllable, and the magnetic particles content is controllable.

Polymerization process for preparing monodispersal organic/inorganic composite nano-microsphere

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Shanghai, China

Hongchen Gu