Tsinghua University

National Yang Ming Chiao Tung University

Hong-Yuan Huang

Da-Jeng Yao

Cheng-Hsien Liu

Shih-Kang Fan

Pei-Yu Chang

Wensyang Hsu

Wen-Syang Hsu

Chang-Hung Tien

Po-Huai Shih

Ting-Ju Yueh

Chi-Fan Chen

Kuo-Wei Chang

Cheng-Yeh Huang

Chin-Jung Li

Po-Yen Tsai

A magnetic bead-based digital microfluidic immunoanalysis device and a method thereof are provided, which includes a lower plate, an upper plate disposed above the lower plate, a separating structure therebetween and a magnet disposed on the upper plate or the lower plate. The lower plate includes a first electrode layer including a plurality of channel electrodes with different sizes. A droplet containing few magnetic beads is adapted to be disposed on the lower plate and corresponding to the channel electrodes. The magnet attracts the magnetic beads to approach to the smaller one of the channel electrodes though a magnetic force, and when a voltage is applied to the first electrode layer, the droplet is divided to a detection portion with the magnetic beads and a waste-liquid portion without the magnetic beads respectively corresponding to the smaller one and the larger one of the channel electrodes through a dual-direction electrowetting-on-dielectric force.

Magnetic bead-based digital microfluidic immunoanalysis device and method thereof

The present disclosure illustrates a platform system for in vitro cell co-cultivation with automatic trapping function. The platform system aims to develop a bio-chip applied in cell culture systems, and has several features. The first feature is that this co-cultivation platform can construct a micro environment suitable for culture of various cells. The second feature is dynamic perfusion. The microfluidic system is used to dynamically replace the culture medium, in order to maintain an appropriate environment for the growth of cells. The third feature is the automatic trapping. The cells to be cultured can be trapped in a suitable location according to the flow resistance, so that the damaged on the cell caused by manual operation can be minimized.

Platform system for in vitro cell co-cultivation with automatic trapping function

Growing community of inventors

Taoyuan, Taiwan

Hong-Yuan Huang