Zymogenetics, Inc.

University of Washington

Bristol-myers Squibb Compnay

Hong Yan Liu

Paul O Sheppard

Kevin M Klucher

Thomas R Bukowski

Dennis L Dong

Lowell J Brady

Chung Chan

Chung-Leung Chan

Bruce L Zamost

Karen S De Jongh

Xiaohu Gao

Jeffrey D Meyer

Susan D Holderman

Susan H Julien

Douglas C Covert

Brian J Reardon

Susan D Julien

Disclosed herein are chimeric proteins that include one or more double stranded nucleic acid binding domains (dsNABD) and one or more polyHis domains, and compositions that further include a therapeutic double stranded nucleic acid and a targeting ligand bound to the therapeutic double stranded nucleic acid, wherein the dsNABD of the chimeric protein is bound to the therapeutic double stranded nucleic acid, and uses of the compositions to treat disease.

Universal protein tag for double stranded nucleic acid delivery

The expression vectors and methods using anexpression system for the large scale production of FGF18 are described. The vectors utilize the FGF18 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in. Using the expression vectors, the FGF18 gene was produced into a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficientstrains, as well as OmpT and fhuA negative strains transformed with an FGF18 expression vector.

FGF18 production in prokaryotic hosts

Homogeneous preparations of IL-28A, IL-28B, and IL-29 have been produced by mutating one or more of the cysteine residues in the polynucleotide sequences encoding the mature proteins. The cysteine mutant proteins can be shown to either bind to their cognate receptor or exhibit biological activity. One type of biological activity that is shown is an antiviral activity.

Method of using IL-29 cysteine mutant to treat hepatitis C and hepatitis B

Polynucleotide encoding an IL-28A polypeptide

Polynucleotide encoding an IL-28A polypepide

Polynucleotide encoding an IL-28B polypeptide

The expression vectors and methods using anexpression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in. Using the expression vectors, the IL-21 gene was produced into a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficientstrains transformed with an IL-21 expression vector.

IL-21 production in prokaryotic hosts

Polynucleotide encoding an il-29 polypeptide

Polynucleotide encoding an IL-29 polypeptide

Growing community of inventors

Seattle, WA, United States of America

Hong Yan Liu