Agency for Science, Technology and Research

Other

Illumina Corporation

Illumina Cambridge Limited

Illumina Singapore Pte. Ltd.

Nitto-Denko Corporation

Hong Xie

Zhiqiang Gao

Fang Xie

Hai Quang Tran

Alexandre Richez

Lewis J Kraft

Sean M Ramirez

Brian Douglas Mather

Xavier Von Hatten

Ludovic Vincent

Zhiping Wang

Jun Wei

Gianluca Andrea Artioli

Julia Morrison

Wei Wei

Edwin Li

Hayden Black

Maxwell Zimmerley

Yuan Hong Yu

Krystal Sly

Innsu Daniel Kim

Sojeong Moon

Ryan Sanford

Wolfgang Knoll

Asmorom Kibrom

Jamie Han

Jakub DOSTÁLEK

An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

Protective surface coatings for flow cells

Compositions and methods for electrochemical detection of an analyte comprising a transition metal compound 

Mediator-modified redox biomolecules for use in electrochemical determination of analyte

A method for the detection and/or quantification of at least one target nucleic acid or target polypeptide in a sample of nucleic acids or polypeptides comprising the steps of: a) providing a sample comprising nucleic acids or polypeptides; b) labeling the nucleic acids or polypeptides with a ligand conjugate, the ligand conjugate comprising a first element binding to the nucleic acids or polypeptides and a second element which is a capture ligand; c) contacting the nucleic acid-ligand conjugates or polypeptide-ligand conjugates with at least one capture probe, the capture probe hybridizing with or binding to at least one target nucleic acid or target polypeptide; d) adding i) an oxidoreductase enzyme, wherein the oxidoreductase enzyme is recognized by the capture ligand, or ii) a complex comprising an oxidoreductase enzyme bound to a capture receptor, the capture receptor capable of binding to the capture ligand; e) adding a redox polymer, the redox polymer binding to the oxidoreductase enzyme, thereby resulting in the transfer of electrons from the enzyme via the redox polymer to an electrode surface; and f) detecting and/or quantifying the presence of the target nucleic acid(s) or target polypeptide(s).

Method and device for detection of nucleic acids and/or polypeptides

A threading intercalator of general formula I:IG-DG-IG-(DG-IG)&#x2003;&#x2003;(I)wherein IG, IG, and IGare the same or different and represent an intercalating group comprising a planar polyaromatic group; wherein DG represents an electrochemical, a chemiluminescent, a catalytic or an electrochemiluminescent detectable group; and wherein n represents 0 or 1. This invention also relates to a process of detecting a double strand nucleic acid molecule using the threading intercalator.

Detectable threading intercalator

Compositions and methods for electrochemical detection of an analyte comprising a transition metal compound  wherein M is a metallic element that can form a coordinate bond to nitrogen; R and R' are coordinated to M at their nitrogen atoms; L is a linking ligand; Z is chlorine or bromine; m can be from 1 to 6 and X is an anion, or combination of anions, that balances the charge m. Also provided are electrochemical tags and methods of detection.

Compositions and methods for electrochemical detection of nucleic acid are disclosed. The compositions comprise electrocatalytic intercalators that bind to double-stranded nucleic acid and oxidize nucleic acid. Methods of detecting nucleic acid in a sample comprise forming double-stranded nucleic acid that is bound by electrocatalytic intercalators wherein the intercalators catalyze oxidation of the nucleic acid at the surface of an electrode.

Determination of nucleic acid using electrocatalytic intercalators

A method of bonding two components by depositing an amorphous and non-hydrogenated intermediate layer () on one of the components () and arranging the components () in spaced relationship with the intermediate layer () therebetween. The method further comprises heating one or both of the components () before bringing the components () into contact. Finally, a voltage is applied to the components () to create a permanent bond between the two components.

Method of wafer/substrate bonding

Disclosed herein are novel high oxidation state metallocene compounds, processes for preparing these compounds and uses of these compounds.

Metallocenes and processes for their preparation

Growing community of inventors

Singapore, Singapore

Hong Xie