Zhongshan Ophthalmic Center, Sun Yat-Sen University

State Grid Corporation of China

Beijing China-Power Information Technology Co., Ltd.

Hong Ouyang

Bo Hu

Liqiong Zhu

Yuxi Liu

Jiakui Zhao

Wenhong Wang

Provided is a method of serum-free culturing corneal limbal stromal stem cells and inducing them to differentiate and form spheres. Also provide is a medium combination for inducing corneal limbal stromal stem cells to form spheres or differentiate into corneal limbal stromal cells in vitro. The serum-free medium combination used herein can provide sufficient nutrients and a good environment required for cell growth and proliferation, can provide a stable in vitro expansion of corneal limbal stromal stem cells and can ensure that the expanded corneal limbal stromal stem cells keep their stemness and specificity. In addition, a system for inducing them to differentiate into corneal limbal stromal cells is successfully built. It can be used in experimental studies of corneal limbal stromal stem cells, cell therapy of corneal lesions and transplant for corneal injury.

Methods of serum-free culturing corneal limbal stromal stem cells and inducing sphere formation and differentiation in vitro

Growing community of inventors

Guangdong, China

Hong Ouyang