Duke University

Colorado State University Research Foundation

Precision Biosciences, Inc.

Homme W Hellinga

James Jefferson Smith

Derek Jantz

Malin J Allert

Loren Lee Looger

June I Medford

Mauricio S Antunes

Kevin J Morey

David J Sloan

David W Conrad

David E Benson

The present subject matter provides urea biosensors as well as compositions, devices, and methods comprising such biosensors.

Urea biosensors and uses thereof

The present subject matter provides biosensors for bicarbonate and calcium as well as compositions, devices, and methods comprising such biosensors.

Bicarbonate biosensors, calcium biosensors, and uses thereof

The present subject matter provides glucose biosensors as well as compositions, devices, and methods comprising such biosensors.

Thermostable glucose biosensors and uses thereof

Provided herein are glucose and galactose biosensors and methods of making and using the same.

Glucose/galactose biosensors and methods of using same

Glucose biosensors and uses thereof

The present subject matter provides lactate biosensors as well as compositions, devices, and methods comprising such biosensors.

Lactate biosensors and uses thereof

Biosensors are made by attaching covalently or non-covalently at least one reporter group to one or more specific positions of a bacterial periplasmic binding protein (bPBP). Upon binding of ligand to the biosensor, there is a change in the signal transduced by the reporter group.

Biosensor

Methods of optimizing mRNA sequences for expression in host cells are provided. Methods of determining the stability of a protein are also provided. Methods of determining the affinity of a ligand for a protein are also provided.

Protein engineering

Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

Method for producing genetically-modified cells with rationally-designed meganucleases with altered sequence specificity

Rationally-designed meganucleases with altered sequence specificity and heterodimer formation

Methods of cleaving DNA with rationally-designed meganucleases

A eukaryotic input circuit: computationally designed receptors, synthetic eukaryotic signal transduction pathways, and a synthetic signal sensitive promoter that allow highly specific transcriptional induction in response to an externally provided ligand is disclosed. The input circuit is able to specifically bind a targeted substance and transmit a signal to the nucleus where transcription of a gene is activated. An output circuit serves as a simple readout system of the substance detected by the input circuit. The readout circuit exemplified here is a degreening circuit which causes plants to turn white. Activation of the degreening circuit can be detected by eye, or remotely with a variety of machines (hand-held, aircraft or satellite based) and is also resettable. When linked the input circuit if operably linked to the output circuit, produces a functional plant detector.

Growing community of inventors

Durham, NC, United States of America

Homme W Hellinga