Sru Biosystems, Inc.

The General Hospital Corporation

X-body, Inc.

The Charles Stark Draper Laboratory, Inc.

Homer Pien

Brian T Cunningham

Peter Li

Bo Lin

Jane Pepper

Jean Qiu

William C Karl

John Gerstenmaier

Derek Puff

Thomas J Brady

Zhuangli Liang

Jeffrey T Borenstein

Mannudeep Kalra

Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.

Optical detection of label-free biomolecular interactions using microreplicated plastic

Performing high-resolution determination of the relative shift of the spectral properties of a biosensor. The shift in the resonance peak of the biosensor is indicative of the amount of material bound to the surface of the biosensor. A preferred biosensor is a Guided Mode Resonant Filter Biosensor (GMRFB). In one aspect of the invention, curve fitting is used to determine the relative location of the spectrum of the unexposed biosensor with respect to those spectra that are altered (e.g., shifted) by the presence of materials bound to the surface of the biosensor. In an alternative embodiment, the cross correlation function is used to detect spectral peak offsets between a reference spectrum and a spectrum measured from an exposed biosensor. In yet another alternative, maximal likelihood estimation techniques are used to determine the spectral shift or offs.

Method and apparatus for biosensor spectral shift detection

In various embodiments, microengineered phantoms with microvascular networks serve as calibration standards for perfusion imaging systems.

Engineered phantoms for perfusion imaging applications

In one aspect, the invention is a method of medical image processing. The method includes receiving data representing a medical image. The method also includes generating the medical image based on a model. The model characterizes the medical image as a composition of at least two components having processing constraints.

Medical image processing

Optical detection of label-free biomolecular interactions using microreplicated plastic sensor elements

Guided mode resonant filter biosensor using a linear grating surface structure

In one aspect, the invention is a method to reduce a blooming effect of a bright object in a medical image generated from a low-dose imaging system. The method includes characterizing an ideal image as a composition of a first component and a second component. The first component is characterized by a first function and the second component is characterized by a second function. The method also includes minimizing the first function and the second function using a smoothing process and generating the medical image based on the minimizing.

Methods are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also relates to optical devices.

Label-free methods for performing assays using a colorimetric resonant reflectance optical biosensor

Label-free high-throughput optical technique for detecting biomolecular interactions

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Andover, MA, United States of America

Homer Pien