Mitsui Chemicals, Inc.

The University of Toyama

Hokkaido Mitsui Chemicals Inc.

Mitsui Petrochemical Industries, Ltd.

National University Corporation University of Toyama

Homare Tabata

Yasuhiro Hara

Yukihito Yukimune

Yosuke Higashi

Chuzo Suga

Kouichi Matsubara

Naoto Ohnishi

Hideki Niimi

Isao Kitajima

Tomohiro Ueno

Hisaaki Yanai

Koh Amano

Hiroshi Minami

Shiroh Hayashi

Masashi Mori

Tomomi Sakai

Disclosed is a thermostable DNA polymerase preparation which can illimitably reduce the risk of false positivity in the detection of a subject microorganism utilizing a gene amplification reaction and therefore enables the selective amplification of DNA for detecting the subject microorganism even when the amount of the subject microorganism is small and therefore the amount of DNA collected therefrom is extremely small, and can be produced at a reduced cost. Also disclosed is a method for quantifying or quantifying/identifying a subject organism to be detected rapidly, conveniently and with high sensitivity using the preparation of the present invention.

Enzyme preparation containing thermostable DNA polymerase, method for producing same, and method for detecting subject organism to be detected

Method of producing a taxane-type diterpene and a method of obtaining cultured cells which produce the taxane-type dilterpene at a high rate

This invention relates to a method of producing a taxane-type diterpene(s) wherein tissues or cells of a plant which produces taxane-type diterpene(s) is cultured in the presence of at least one selected from the group consisting of jasmonic acids, compounds containing a heavy metal, complex ions containing a heavy metal, heavy metal ions, amines and antiethylene agents, a method of producing a taxane-type diterpene wherein the tissues or the cells of the plant are cultured by controlling the oxygen concentration in a gas phase in a culture vessel to less than the oxygen concentration in the atmosphere from the initial stage of the culture, or by controlling the dissolved oxygen concentration in a fluid medium which is in contact with the tissue or the cell to less than the saturated dissolved oxygen concentration at that temperature from the initial stage of the culture, a method of producing a taxane-type diterpene wherein the tissue or the cell of the plant is cultured in a culture vessel, while oxygenic gas containing 0.03-10% of carbon dioxide is used as aeration gas to be introduced to the vessel, and a method of obtaining highly productive cultured cells for the taxane-type diterpene wherein cultured cells of the plant which produces the taxane-type diterpene are separated into a plurality of layers according to the difference in their specific gravities, and the cells contained in at least one layer are cultured, then highly productive cultured cells for the taxane-type diterpene are selected from among those cultured cells. The present invention allows industrial production of a taxane-type diterpene such as taxol which is useful as a therapeutic agent for cancer.

Method of producing a taxane-type diterpene and a method of obtaining

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Kuga-gun, Japan

Homare Tabata