Ortho-clinical Diagnostics, Inc

Johnson & Johnson Clinical Diagnostics, Inc.

Holly L Groth

Brian A Snyder

Paul B Contestable

Harold C Warren

John L Daiss

Shirley Y Lynn

Elizabeth A Grogan

Lisa D Sprague

Reduced cortisol conjugates

Specific binding ligands can be detected with an assay which utilizes an immobilized receptor for the ligand, an immobilized reporter enzyme, an inhibitor antibody and a a water-soluble conjugate of the ligand and an anti-inhibitor antibody. Both antibodies are specific for the reporter enzyme, but the antibodies affect enzymatic activity differently. The inhibitor antibody effectively shuts down the activity of the reporter enzyme when it is complexed thereto. The anti-inhibitor antibody binds to the reporter enzyme, does not affect the enzymatic activity, but prevents the binding of the inhibitor enzyme. This assay provides a direct correlation of the generated signal to the target specific binding ligand.

Specific binding assay using enzyme inhibitor and anti-inhibitor

Growing community of inventors

Hamlin, NY, United States of America

Holly L Groth