Genentech, Inc.

F. Hoffmann-la Roche Ag

Hok Seon Kim

James A Ernst

Christoph Spiess

Yan Wu

Daniel G Yansura

Yongmei Chen

Junichiro Sonoda

Scott Stawicki

Yu-Ju G Meng

Angie Grace Yee

Ingrid Kim

James Ernst

Joanne Hongo

Diana Ronai Dunshee

The presently disclosed subject matter provides antibodies that bind KLB and FGFR1, and methods of using the same. In certain embodiments, an antibody of the present disclosure includes a bispecific antibody that binds to an epitope present on FGFR1 and binds to an epitope present on KLB.

Host cell comprising nucleic acids encoding bispecific antibodies binding to beta-klotho and fibroblast growth factor receptor 1 and antibody production

The instant disclosure provides antibody fragments (e.g., Fab and F(ab')) having reduced or no reactivity towards pre-existing anti-hinge antibodies (AHA) and compositions comprising such antibody fragments, as well as methods of making and using such antibody fragments and compositions.

Hinge modified antibody fragments and methods of making

Nucleic acids encoding bispecific antibodies binding to beta-Klotho and fibroblast growth factor receptor 1

Methods of treatment with bispecific antibodies binding to beta-klotho and fibroblast growth factor receptor 1

Bispecific antibodies binding to beta-klotho and fibroblast growth factor receptor 1

Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents, as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts.

Methods for producing soluble membrane-spanning proteins

Methods for producing soluble membrane spanning proteins

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San Francisco, CA, United States of America

Hok Seon Kim