Samyang Genex Corporation

Samyang Biopharmaceuticals Corporation

Samyang Genexbio Corporation

Inbionet Corporation

Ho-Joon Choi

Moon-Suk Kim

Jin-Suk Cho

Bong-Kyu Song

Sang-Hyun Pyo

Jeong-Hwan Yun

Jai-Young Song

Seung-Suh Hong

Sang-Ic Kim

Hyung-Kyoon Choi

Chang-Heon Kim

Jin-Ah Kim

Hyun-soo Lee

Jin-Hyun Kim

Roy William Stahlhut

Tom Lee Adams

Hyun-Soo Lee

Jun-Seog Song

Jong-Won Yoon

Hong-Rak Kim

Bon-Tag Koo

Eunsoo Choi

Moon-Seok Chang

Joo-Sun Son

Kee-Don Park

Jung-Ki Lee

Key-Jung Kang

Daesang Lee

Do-Young Yum

Chul-Soo Shin

Mi-Kyoung Lee

The present invention relates to a method of producing secondary metabolites with a high yield using plant cell culture, and a medium for the production of secondary metabolites. More specifically, the method of the present invention is characterized in that the plant cell culture is conducted by adding a saccharide mixture to the culture medium as a carbon source, to increase the productivity of the secondary metabolites. For example, the present invention establishes the method of increasing the productivity of secondary metabolites and shortening the culture time by the use of the mixture of glucose and fructose in the plant cell culture, thereby contributing in producing useful secondary metabolites on an industrial scale using plant cell culture.

Mass production of secondary metabolite in plant cell culture by treatment of saccharide mixture in medium

Provided is a method for preparing a taxane derivative, comprising: carrying out condensation of a phenylisoserine derivatives having a protective group introduced thereto or a mixture of isomers thereof, as a side chain, with a baccatin III derivative or 10-deacetyl-baccatin III derivative to obtain a mixture of isomers; separating the isomers via chromatography; and carrying out a reversion of the stereochemical structure of a selectively separated isomer, which is suitable for producing a taxane derivative in a large scale with high yield.

Method for preparing taxane derivatives

A method for preparing highly pure anhydrous crystalline docetaxel is provided. The method for preparing highly pure anhydrous crystalline docetaxel enables preparation of anhydrous crystalline docetaxel that has purity of 99.5% or more, and is useful as an anticancer agent due to remarkably low residual solvent content compared to the standard of residual solvents in drugs.

Method for preparing highly pure anhydrous crystalline docetaxel

This invention is directed to a method for the preparation of docetaxel having an amorphous, anhydrous crystalline, or hydrated crystalline structure. In accordance with the invention, highly pure amorphous, anhydrous crystalline, or hydrated crystalline docetaxel can be produced with a high yield by using a solvent precipitation method, colloid formation method, etc.

Method for preparation of amorphous, anhydrous crystalline or hydrated crystalline docetaxel

The present invention relates to a method of producing corosolic acid by using plant cells that produce corosolic acid. More particularly, the present invention relates to a method of producing corosolic acid by using plant cell suspension cultures comprising the steps of: inducing a callus from a tissue of a plant producing corosolic acid; preparing a cell line capable of being cultured in liquid culture medium from the induced callus; culturing the cell line in a suspension culture; and isolating corosolic acid from the culture solution. The present invention has advantage of maximizing productivity by utilizing two-stage culture, treatment with inducing agent, and high cell-density culture in the suspension culture of plant cells producing corosolic acid.

Method for production of corosolic acid in suspension culture of plant cells

This invention is directed to a method for efficiently separating and purifying 13-dehydroxybaccatin III and 10-deacetylpaclitaxel with a high purity of 90% or higher purity, preferably 99.5% or higher purity, from taxane-containing materials such asspecies, and the thusly obtained highly pure 13-dehydroxybaccatin III and 10-deacetylpaclitaxel can be used as semi-synthetic precursors of paclitaxel and/or docetaxel that are anticancer agents.

Method for separation and purification of 13-dehydroxybaccatin III and 10-deacetylpaclitaxel from taxans-containing materials

This invention relates to methods for purification of paclitaxel from a paclitaxel-containing material. The method comprises the following steps: (a) extracting a paclitaxel-containing material with an organic solvent to obtain an extract, and concentrating the extract; (b) adding the concentrate with an organic solvent which is not mixed with water to separate an organic solvent phase and then concentrating; (c) subjecting the concentrate to normal phase chromatography to obtain an eluate; (d) dissolving the eluate in an acetone or dichloromethane followed by adding pentane or hexane to form a precipitate; and (e) subjecting the precipitate to high performance liquid chromatography. According to the method of the present invention, paclitaxel of over 99.5% purity can be easily obtained from agenus plant with a high yield.

Method for purification of paclitaxel from paclitaxel-containing materials

The present invention describes a wastewater treatment method by a microorganism decomposing Tetramethyl Ammonium Hydroxide (TMAH) which, utilized in etching the surface of silicone chip in semiconductor manufacturing process, is toxic and hard to decompose. The present invention provides novel microorganisms capable of decomposing TMAH. Also, the present invention provides a treatment method for wastewater containing TMAH, using the microorganisms. The present invention is useful in industrial field as an environmental friendly wastewater treatment method by decomposing over 90% of TMAH, one of environmental contamination materials in the wastewater of semiconductor factory.

Strain for decomposing TMAH, and method of wastewater treatment using the same

The present invention relate to a method to increase the production level of paclitaxel by changing the temperature during the plant cell culture. According to the present invention, the production of the paclitaxel comprises the following procedure: (i) cultivating the Taxus genus plant cells in a medium at ca. 20 to 25&deg; C.; and (ii) when growth of the plant cells has progressed sufficiently, changing the cultivation temperature to ca. 26 to 32&deg; C. to continue the culture. The present invention comprises also the method of increasing the paclitaxel production by inoculating the cells at a high initial concentration and by increasing the saccharide concentration in the medium: According to the present invention, paclitaxel can be mass-produced conveniently and therefore has an industrial application.

Mass production of paclitaxel by changing the temperature of the medium during the plant cell culture

The present invention provides a method for mass production of taxol which comprises adding sugar into a culture medium containing sugar in the course of semicontinuous culture of Taxus genus plant cell, and a universal method for mass production of taxol which comprises culturing Taxus genus plant cell in a culture medium containing sugar and AgNO,as well. In accordance with the present invention, large amount of taxol can be produced with a high yield, simply by manipulating the culture condition of Taxus genus plant cell culture.

Production of taxol from taxus plant cell culture adding silver nitrate

Growing community of inventors

Daejeon, South Korea

Ho-Joon Choi