University of South Florida

The United States Government As Represented by the Department of Veterans Affairs

University of Florida Research Foundation, Incorporated

Hla Yee Win

Mildred Enid Acevedo-Duncan

Raoul Salup

David A Ostrov

Diondra Denise Hill

Shraddha R Desai

Prajit P Pillai

A method of detecting prostate tumorigenesis in a subject, the method including the steps of (a) obtaining a sample from the prostate of the human subject, (b) detecting quantitatively or semi-quantitatively in the sample a level of expression for PKC-&#x3b9; and (c) comparing the expression level in (b) to a level of expression in a normal control, wherein overexpression of PKC-&#x3b9;, with respect to the control, indicates the presence of prostate cancer in the subject. The present invention is based upon the discovery that PKC-&#x3b9; levels are elevated during prostate tumorigenesis. Furthermore, the proliferation rate of the tumor correlates with the level of PKC-&#x3b9;. The invention also provides methods of treating prostate cancer by administering to the subject a compound that inhibits the expression of PKC-&#x3b9;. The compound can be a small interfering RNA (siRNA) molecule.

Prostate carcinogenesis predictor

PKCiota inhibitor 1H-imidazole-4-carboxamide, 5-amino-1-[2,3-dihydroxy-4-[(phosphonooxy)methyl]cyclopentyl]-,[1R-(1&#x3b1;,2&#x3b2;,3&#x3b2;,4&#x3b1;)], (ICA-1), targets a unique sequence (amino acid residues 469-475) in the catalytic domain of PKCiota and inhibits PKCiota activity. The data shows suprising and unexpected ability of ICA-1 to selectively inhibit the proliferation of cells that overexpress PKCiota.

Method of selectively inhibiting PKCiota

Growing community of inventors

Tampa, FL, United States of America

Hla Yee Win